Extraction method of anemone a and preparation method of lipid microsphere preparation

An extraction method, the technology of anemone, which is applied in the field of medicine, can solve the problems of unseen and low yield of anemone A, achieve the effects of no hemolysis, overcome the unstable chemical properties of the drug, and reduce the dosage

Inactive Publication Date: 2011-12-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention provides an extraction process of anemone A and a preparation process of lipid microsphere preparation thereof, so as to solve the shortcoming of low yield of anemone A in the prior art. Problems with Liposphere Formulations of Drugs

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  • Extraction method of anemone a and preparation method of lipid microsphere preparation
  • Extraction method of anemone a and preparation method of lipid microsphere preparation

Examples

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Embodiment 1

[0045] Take 1000g of both tips, add 3000ml of 95% ethanol, soak for 48 hours, add 2700ml of water, reflux extraction for 2 hours, filter, distill under reduced pressure until there is no alcohol smell (for standby), decoct the dregs with 5700ml of water twice, each time 3 hours, filter, the filtrate is combined with the extract of recovered ethanol, concentrated to 5000ml, filtered, put on a D101 macroporous resin column, and eluted with water, 20% ethanol, and 40% ethanol of 4 times the column volume respectively, discarding the water washing solution , collect ethanol eluents of different concentrations respectively, reclaim ethanol respectively, and concentrate to 3000ml respectively, add 3000ml NaOH solution with pH value of 13 to the concentrated solution of 20% ethanol eluent, heat and boil for 8 hours, add pH value An appropriate amount of HCl solution of 1, adjust the pH to 7, combine with the concentrated solution of 40% ethanol eluent, add 1% activated carbon for deco...

Embodiment 2

[0047] Take 1000g of two tips, add 3000ml of 95% ethanol, soak for 48 hours, add 2700ml of water, reflux extraction for 2 hours, filter, extract the dregs with 50% ethanol twice, each time for 2 hours, filter, the filtrate and the above ethanol extract Combine, recover ethanol, concentrate to 10000ml, filter, put on D101 macroporous resin column, elute with 5 times column volume of water, 15% ethanol, 35% ethanol respectively, discard the water wash, collect ethanol with different concentrations to elute solution, reclaim ethanol respectively, and concentrate to 2000ml respectively, add 1000ml NaOH solution with a pH value of 13 to the concentrated solution of the 15% ethanol eluent, heat and boil for 8 hours, add an appropriate amount of HCl solution with a pH value of 1, and adjust the pH to 7. Combine with the concentrate of 35% ethanol eluent, decolorize through ZTC resin column, recover ethanol from the decolorized solution, concentrate, dry, add methanol to dissolve, put ...

Embodiment 3

[0049] Take 1000g of two tips, add 5000ml of 50% ethanol, soak for 36 hours, add 1000ml of water, reflux extraction for 3 hours, filter, extract the dregs with 40% ethanol twice, each time for 3 hours, filter, the filtrate and the above ethanol extract Combine, recover ethanol, concentrate to 10000ml, filter, put on AP8 macroporous resin column, elute with 4 times column volume of water, 1% ethanol, 30% ethanol respectively, discard the water washing solution, collect ethanol with different concentrations to elute solution, reclaim ethanol respectively, and concentrate to 2000ml respectively, add 1000ml NaOH solution with a pH value of 8 to the concentrated solution of 1% ethanol eluent, heat and boil for 1 hour, add an appropriate amount of HCl solution with a pH value of 1, and adjust the pH to 6. Combine with the concentrated solution of 30% ethanol eluent, decolorize through ZTC resin column, recover ethanol from the decolorized solution, concentrate, dry, add methanol to d...

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Abstract

The invention provides an extraction method of anemone A and a preparation method of a lipid microsphere preparation. The method is based on the medicinal material of the two ends of the tip, soaked in high-concentration alcohol, added with water, extracted, concentrated, passed through a macroporous resin, and selectively It is obtained by hydrolysis, silica gel column chromatography, recrystallization and purification; and anemone A is used as the active ingredient, plus commonly used pharmaceutical oils for injection or oral use, emulsifiers, auxiliary emulsifiers, stabilizers, isotonic agents, Lipid microsphere preparations prepared from antioxidants, water for injection or pharmaceutical water. The method minimizes the activity of the enzyme, increases the yield of anemone A by selectively hydrolyzing saponins containing more than trisaccharides, and obtains anemone A, a monomer component with strong anticancer activity , the process is simple to operate and suitable for industrial production; and the lipid microsphere preparation prepared by this method has good stability, and the physical and chemical properties have no obvious change after autoclaving. The preparation meets the relevant requirements for clinical use.

Description

technical field [0001] The present invention relates to a kind of saponin extraction and preparation method and lipid microsphere preparation preparation technology thereof, more specifically, the present invention relates to a kind of saponin, the extraction and preparation method of anemone A from traditional Chinese medicine two-headed tip and The lipid microsphere preparation and the preparation method thereof belong to the field of medicine. Background technique [0002] Anemone A (chemical name 3-O-[α-L-rhamnopyranose-(1→2)-β-D-glucopyranose-(1→2)-α-L-arabinopyranose Sugar] oleanolic acid) is a main active ingredient in traditional Chinese medicine. The structural formula of anemone A is as follows: [0003] [0004] Anemone A is a colorless powder, melting point: 242-244°C (decomposition); molecular formula C 47 h 76 o 16 ·H 2 O; [α] D 2 -8(c, 0.8, CH 3 Oh) [0005] Two-headed tip, also known as cymbal cyperus, multi-covered anemone, is the rhizome of red...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61K9/16A61J3/00A61P35/00
Inventor金向群李海燕丁志英韩丛成杨锦竹郝秀华管清香
OwnerJILIN UNIV