Monascus purpureus orotic acid-5'-phosphate decarboxylase gene and uses thereof

Abstract

The invention relates to monascus orotic acid-5'-phosphoric acid decarboxylase genes and application thereof, belonging to the technical field of microbe gene. The invention comprises the monascus orotic acid-5'-phosphoric acid decarboxylase genes with an SEQ ID NO.1 sequence or homologous compounds which have at least 80 percent homology with the SEQ ID NO.1 sequence and amino acid sequences coded by the genes or the homologous compounds; in a gene construction, the genes or the homologous compounds are effectively connected with one or more regulating signals; and the invention also comprises the application of the genes or the homologous compounds in monascuses as a selective marker. The invention constructs uracil auxotrophs monascuses with stable inheritance by the way of genetic engineering, and the bacterial strains not only are convenient for the genetic transformation and the screening of recombination bacterial strain but also maintain the physiological-biochemical characteristics of wild type bacterial strains, are favorable for the culture of the recombination bacterial strains and the effective expression of foreign proteins, and have relatively high industrial application value.

Description

technical field [0001] The invention belongs to the technical field of microbial genes, and relates to an orotate-5'-phosphate decarboxylase gene with the sequence of SEQ ID NO.1 or its homologue and its application. technical background [0002] Monascus (Monascus, also known as Monascus) has been used in food and traditional Chinese medicine for thousands of years. Modern research has found that Monascus can produce a variety of physiologically active substances. These physiologically active substances are mainly metabolites of Monascus, including antibacterial active substances (such as: monascorubrin), hypotensive substances (such as: γ-aminobutyric acid, acetylcholine), hypoglycemic substances, anti-cancer substances, antioxidant substances, Ergosterol, long-chain fatty acids, cholesterol synthesis inhibitors (eg monacolin), etc. Therefore, the research, development and application of Monascus have been highly valued in recent years. [0003] In order to increase the ...

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Problems solved by technology

The domestic gene expression system with antibiotic resistance as the selection pressure has the following defects: 1. Antibiotics need to be added in production to ensure the stable expression of exogenous genes, which increases production costs; 2. The use of antibiotics is a genetic engineering product An important source of potential tra

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