Deletion mutant of maize phosphatidylinositol synthase gene promoter p-zmpis and its application

Abstract

The invention discloses a deletion mutant of maize phosphatidylinositol synthase gene promoter P-ZmPIS. The deletion mutant is named as a PZ7 promoter and is a continuous sequence comprising 467 nucleotides, close to an open reading frame, of the maize phosphatidylinositol synthase gene promoter P-ZmPIS, wherein the nucleotide sequence of the P-ZmPIS promoter is shown in SEQ ID No.1, and the nucleotide sequence of the PZ7 promoter is shown in SEQ ID No.2. The invention also discloses application of the promoter PZ7 in plant breeding for stress resistance, experiments show that PZ7 promoters promote the downstream genes to have NaCl and drought stress-induced characteristics in transformed cells, the deletion mutant has important application values in stress resistance genetic engineering breeding of salt-tolerant and drought-resistant plants.

Description

technical field

[0001] The invention belongs to the technical fields of molecular biology and plant genetic engineering, and in particular relates to a deletion mutant PZ7 of a corn phosphatidylinositol synthetase gene promoter P-ZmPIS and application thereof. Background technique

[0002] Soil drought and salinization have seriously affected plant growth and development, and have become important factors affecting agricultural production. In recent years, the rapid development of molecular biology technology and the in-depth study of functional genomics of Arabidopsis, rice, corn and other model plants have provided new ideas and methods for breeding new varieties of drought-resistant and salt-tolerant crops. The biological process of crops coping with drought and unfavorable soil salinization and promoting yield is regulated by multiple key genes, discovering and cloning key functional genes and promoters of stress tolerance, and using transgenic technology to cultivate dr...

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