Stevia rebaudiana tissue culture method and culture medium thereof

Abstract

The invention relates to a stevia rebaudiana tissue culture propagation method and a culture medium of the method, and particularly relates to a tissue culture rapid propagation method for directly forming a bud by using a stevia rebaudiana leaf as an explant. The stevia rebaudiana tissue culture method comprises the following steps: (1), collecting the explant; (2), disinfecting and inoculating; (3), performing thickening induction-culturing; (4), budding and seedling; (5), transferring and grafting, and rooting; and (6), hardening and transplanting. According to the stevia rebaudiana tissue culture method provided by the invention, materials are easily available, a plurality of seed sources can be rapidly propagated, the explant tissue culture has no culture phase of callus cell proliferation and differentiation, thus the culture time is shortened, extremely high reproduction rate is achieved; the stevia rebaudiana tissue culture propagation method is easy to operate and is capable of realizing large-scale production; and the operation of researches such as genetic transformation is facilitated in a culture process. The culture medium provided by the invention ensures that the leaf is capable of reaching the expected culture target in each phase.

Description

technical field [0001] The invention relates to a tissue culture and propagation method of stevia rebaudiana and a culture medium thereof, in particular to a tissue culture rapid propagation method and a culture medium thereof in which stevia leaves are used as explants to directly form buds. Background technique [0002] Asteraceae Stevia Stevinrebaudiann, Bertoni, hereinafter referred to as Stevia, is a perennial herb of the Asteraceae Stevia genus native to Paraguay, South America. Because the steviol glycosides contained in Stevia can control blood sugar, lower blood pressure, promote metabolism and treat Diabetes, obesity, regulating gastric acid, recovery of nerve fatigue, and stable physical and chemical properties, non-fermentable characteristics, its products have a relatively long shelf life, especially suitable as sweeteners for patients with diabetes, hypertension and other diseases, Health product additives, etc., have a broad market prospect and application ran...

AI Technical Summary

Problems solved by technology

However, the method of seed propagation has a low survival rate; and the cutting propagation leads to quality degradation, and these two propagation methods take a long time and require a large amount of materials such as stem tips, stem segments with axillary buds, and seeds.