Nucleic acid blot membrane-transferring device

A technology of membrane transfer and imprinting, which is applied in the field of nucleic acid imprinting membrane transfer devices to achieve the effect of improving stability

Inactive Publication Date: 2009-09-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pressure plate moves up and down abov

Method used

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  • Nucleic acid blot membrane-transferring device
  • Nucleic acid blot membrane-transferring device

Examples

Experimental program
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Embodiment Construction

[0020] Such as figure 1 As shown, the nucleic acid imprinting transfer device of the present invention includes a transfer tank 1 for holding a solution, a loading platform 2 arranged on the transfer tank 1 , a pressure plate 3 , a counterweight 4 and a slide bar 5 .

[0021] The film transfer tank 1 is a cuboid container, and four fixing platforms 7 for fixing the slide bars 5 are arranged on four corners in the film transfer tank 1 .

[0022] The fixed table 7 is provided with a screw hole, and the fixed end 6 of the slide rod 5 is provided with a corresponding external thread, and the fixed end 6 is threaded into the screw hole with the through hole. Not only the detachable threaded connection can be used between the slide bar 5 and the fixed table 7, but also other connection methods such as interference fit between the fixed end 6 and the fixed table 7 or welding between the fixed end 6 and the fixed table 7 can be used.

[0023] The bottom of the film transfer tank 1 is...

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PUM

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Abstract

The invention discloses a nucleic acid blot membrane-transferring device which comprises a membrane-transferring trough used for containing solution, wherein the upper part of the membrane-transferring trough is provided with a carrying platform, a pressure plate which can up and down move and is provided with a balance weight is arranged above the carrying platform, and the pressure plate is connected with the membrane-transferring trough by a sliding rod. The pressure plate can move up and down above the carrying platform along the sliding rod. The nucleic acid blot membrane-transferring device has simple structure and convenient operation, and can effectively improve the success ratio of the blot hybridization membrane-transferring experiments.

Description

technical field [0001] The invention relates to a membrane transfer device, in particular to a nucleic acid imprint transfer membrane device used for nucleic acid molecule hybridization experiments. Background technique [0002] In the nucleic acid molecular hybridization (Southern blotting, Northern blotting) experiment, there is a link that needs to transfer the nucleic acid molecular imprint in the agarose gel to the nitrocellulose membrane. [0003] However, there is no corresponding special membrane transfer device in the current experimental operation, so when carrying out the experiment in this link, it is often done with some relatively simple utensils, such as an electrophoresis tank, a glass plate and weights, etc. the process. [0004] During the experiment, filter paper, gel, nitrocellulose membrane, filter paper, and multi-layer absorbent paper are stacked on the loading platform in the electrophoresis tank in sequence. Then press the glass plate on the absorb...

Claims

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Application Information

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IPC IPC(8): C12M1/12
Inventor 丁鸣
Owner ZHEJIANG UNIV
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