Humanized single anti-Hu-ScFv18 light and heavy chain variable region gene and coding polypeptide thereof and application thereof

A technology of hu-scfv18 and gene encoding, applied in application, gene therapy, genetic engineering, etc.

Active Publication Date: 2009-10-07
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

It can be seen from this that there are few similar products of immunohistochemical kits at home and abroad, and there is no product that uses HAb18G / CD147 as a cancer marker for the development of diagnostic reagents.

Method used

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  • Humanized single anti-Hu-ScFv18 light and heavy chain variable region gene and coding polypeptide thereof and application thereof
  • Humanized single anti-Hu-ScFv18 light and heavy chain variable region gene and coding polypeptide thereof and application thereof
  • Humanized single anti-Hu-ScFv18 light and heavy chain variable region gene and coding polypeptide thereof and application thereof

Examples

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Embodiment Construction

[0025] Cloning of light and heavy chain variable region genes of murine HAb18 monoclonal antibody

[0026] The cell line used is a high-affinity, high-specificity mouse-derived anti-human hepatocellular carcinoma monoclonal antibody HAb18 hybridoma cell line obtained by the Cell Engineering Research Center of the Fourth Military Medical University using a traditional fusion method (biological material deposit certificate: Biological Classification and naming of materials: anti-human liver cancer monoclonal antibody HAb18 hybridoma cell line, depository unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms, address: Zhongguancun, Beijing, China, date of deposit: December 6, 1999, deposit number: CGMCCNO.0426), the antibody has passed the requirements of the "Human Mouse-derived Monoclonal Antibody Preparation and Quality Control Key Points" issued by the China Institute for the Control of Pharmaceutical and Biological Products. . The tot...

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Abstract

The present invention relates to a humanized single anti-Hu-ScFv18 light and heavy chain variable region gene for resisting HAb18G / CD147 and A coding polypeptide thereof and application thereof. The invention uses a set of designed primers to humanizedly reconstruct the anti-HAb18G / CD147 single light and heavy chain variable region gene excreted from HAb18 hybridoma, and the humanized single anti-Hu-ScFv18 heavy chain and light chain variable region gene may encode a correct antibody variable region. Based on the cloned anti-HAb18G / CD 147 humanized single anti-Hu-ScFv18 light and heavy chain variable region gene, it is capable of constructing and expressing various small molecule genetic engineering antibodies such as single-chain antibody, jogged antibody, Fab antibody and the like against the HAb18G / CD 147 molecule by a genetic engineering method, so as to be used for diagnosis and treatment of hepatocarcinoma.

Description

technical field [0001] The present invention relates to the light and heavy chain variable region genes of anti-HAb18G / CD147 humanized monoclonal antibody Hu-ScFv18 and polypeptides encoded by the genes, and the use of the genes and polypeptides in the preparation of drugs for diagnosis and treatment of tumors Applications. Background technique [0002] Malignant tumors are currently one of the main killers that seriously affect human health and threaten human life. According to the annual report of the World Health Organization (WHO), 7.6 million people died of malignant tumors (cancer) in the world in 2005. In recent years, research on the etiology and clinical diagnosis and treatment of cancer has made great progress, but the mortality rate of cancer has not been effectively curbed. Cancer is a genetic disease, its occurrence and evolution involve multiple genes and go through multiple different stages, and is related to the regulation disorder of multiple pathways rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/13C07K16/30A61K48/00A61P35/00C12Q1/68
Inventor 陈志南杨向民李郁张征
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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