Flounder tetraplont fry batch induction method

A tetraploid, batch technology, applied in biochemical equipment and methods, fish farming, climate change adaptation, etc., can solve problems such as difficult large-scale promotion and application, inability to establish breeding colonies, and low efficiency of triploid induction , to achieve the effects of high induction efficiency, neat ploidy, and strong practicability
CN101595849BInactive Publication Date: 2011-02-23YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2011-02-23
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a flounder tetraplont fry batch induction method comprising an induction method and an identification method of tetraplont fries; wherein, the induction method comprises collection and insemination of unfertilized ovum, and determination of oosperm chromosome doubling starting time, hydrostatic pressure shock pressure and processing time, and the identification method comprises ploidy determinator identification and chromosome analysis identification. The method firstly builds a method of inducing the tetraplont fries of flounder, such as paralichthysolivaceus and the like by hydrostatic pressure, screens hydrostatic pressure processing starting time and hydrostatic pressure shock conditions for inhibiting first merogenesis and carrying out chromosome doubling, andbuilds a tetraplont fry batch induction method. Tetraplont fries obtained with the method have high induction rate of 90%; the method of the invention has the characteristics of advancing, high efficiency, accuracy and reliability, and has important application value in fish polyploidy induction and sterile offspring seed production, and has wide promotion and application prospect in fish cultivation and breeding.
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Description

Technical field:

[0001] The invention belongs to aquatic genetic breeding technology, and relates to a method for inducing tetraploid of flounder and flounder by physical means. Background technique:

[0002] Flounder, turbot and star flounder are important marine fishes in my country. At present, the annual output value of flounder and flounder in my country is close to 10 billion yuan. However, because many flounder and flounder are sexually mature relatively early, and the gonad development affects the growth and meat quality of the fish and reduces the quality of the product, research on the sterile technology of these fish has been carried out, and sterile populations have been developed, which is very important for improving the quality of these fish. The breeding output and commodity value of species are very important. In addition, more and more types of marine flounder and flounder are being introduced into my country, for example, desert flounder and Atlantic flou...

Claims

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