Non-linear spectral imaging detecting device with high contrast and high resolution

Abstract

The invention relates to a non-linear spectral imaging detecting device with high contrast and high resolution. The non-linear spectral imaging detecting device with high contrast and high resolution is characterized in that the light emitted from a laser is incident upon a scanning mirror group of a scanner via a bicolor spectroscope; the light beam through the scanning mirror group is focused on a detecting sample by an objective lens; the laser light and signal generated by the detected sample are collected by the same objective lens in opposite direction and then are incident upon a beam splitting lens 1 by the scanning mirror group and the bicolor spectroscope; the emitted signal light is divided into two paths; one path enters the spectrograph, the spectral distribution with high resolution of the detected sample is displayed by a computer; the other path divides the signal into three paths again via a beam splitting lens 2 and a beam splitting lens 3; the three paths are respectively connected to photomultiplier detectors 1, 2, 3 so that the emitted light signal is converted to the electric signals A, B and C; the electric signals A, B and C are respectively connected to the input ends of the computer; the imaging with high contrast of different components having different spectral ranges in the detecting sample is displayed by the computer; the device can realize the non-linear spectral imaging detection with high contrast and high resolution for different components with different spectral ranges of the detecting sample.

Description

technical field [0001] The invention relates to a high-contrast, high-resolution nonlinear spectral imaging detection device. Background technique [0002] Spectral imaging technology based on nonlinear optical effects such as harmonics and multiphoton excited fluorescence generated by the interaction between femtosecond laser and biological tissue, combines the advantages of multiphoton microscopy and spectral measurement technology, and can simultaneously obtain the intrinsic composition of tissues Therefore, it has the advantages of high sensitivity and high spatial resolution imaging of tissue microstructure, low lethality to biological tissue, deep imaging depth and fast, real-time and accurate positioning. Since many internal components of biological tissues can generate strong autofluorescence and harmonic signals without external molecular probes, such as elastin, keratin, flavoprotein, NAD(P)H, etc., can all be generated by two-photon excitation Autofluorescence, a...

AI Technical Summary

Problems solved by technology

At present, the detection of nonlinear spectral imaging based on the harmonic wave and multiphoton excitation fluorescence effect generated by the interaction between femtosecond laser and biological tissue mainly adopts two methods. First, the detector consists of a high-quality reflective grating and multiple Composed of a photomultiplier tube array, due to the limitation of the number of photomultiplier tubes, the spectral resolution measured by this detector is only about 10nm, and because the working conditions of each photomultiplier tube in the photomultiplier tube array cannot be independent It is difficult to obtain high-contrast imaging of different components in the detection sample with different spectral ranges; the second is that the detector is composed of two dispersive prisms and a CCD camera. The resolution of this method has been improved and can reach about 2nm , but the imaging contrast of different components in the detection sample with different spectral ranges is low

Images