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Method for measuring haematoxylin content of sapanwood

A technology for quantitative determination of hematoxylin, applied in the direction of material electrochemical variables, etc., can solve the problems of polyphenols being splashy and difficult to quantify accurately, and achieve a good extraction recovery effect

Inactive Publication Date: 2012-10-03
JIANGNAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

It itself is a kind of mixture with very similar structure and properties, and the polyphenols are relatively splashy and prone to condensation or degradation, so it is difficult to accurately quantify them
Although there are dozens of polyphenol quantification methods, these methods are relative, and almost none of them can be applied to all determinations. The determination method and related test conditions must be selected according to the actual situation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The hematoxylin is crushed into 60-mesh granules, 10 g of the sample is taken and the extract (water solution containing 5% ethanol by mass fraction) is added to 50 mL, and extracted by ultrasonic treatment for 5 minutes. The frequency of the ultrasonic generator is 20 kHz, and the power is 100 W. The extraction temperature was 20°C. The residue was extracted 3 times again, and the extracts were 50mL, 30mL, and 20mL respectively. The extracts were combined, and the extract was filtered and then adjusted to 200mL for electrochemical determination.

[0015] Take hematoxylin standard products of different quality, dissolve them in the buffer solution of pH=3, use graphite electrode as working electrode, platinum electrode as counter electrode, and calomel electrode as reference electrode, at a scanning speed of 30mV / s, Measure its cyclic voltammetry curve, the highest peak position of the oxidation peak is at 0.94V, and the highest peak position of the reduction peak is a...

Embodiment 2

[0019] The hematoxylin is crushed into 60-mesh granules, 10 g of the sample is taken and the extract (water solution containing 30% ethanol by mass fraction) is added to 50 mL, and extracted by ultrasonic treatment for 30 minutes. The frequency of the ultrasonic generator is 80 kHz, and the power is 400 W. The extraction temperature is 300C. The residue was extracted 3 times again, and the extracts were 50mL, 30mL, and 20mL respectively. The extracts were combined, and the extract was filtered and then adjusted to 200mL for electrochemical determination.

[0020] Take hematoxylin standard products of different qualities, dissolve them in the buffer solution of pH=9, use graphite electrode as working electrode, platinum electrode as counter electrode, and calomel electrode as reference electrode, at a scanning speed of 90mV / s, Measure its cyclic voltammetry curve, the highest peak position of the oxidation peak is at 0.98V, and the highest peak position of the reduction peak i...

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Abstract

The invention provides a method for quantitatively measuring haematoxylin content of sapanwood by the electrochemical method. The method is characterized in that: according to the physical-chemical properties of the haematoxylin, the pre-treatment of samples adopts the ultrasonic treatment-ethanol and water mixed solvent extraction method; the extracting solution uses a graphite electrode as an anode, a platinum electrode as a cathode, and a calomel electrode as a reference electrode; and in a buffer solution with a pH value of 3 to 11, at a temperature of between 20 and 80 DEG C and a scanning speed of 30 to 60 mv / s, the response signal is measured. By the method, the total haematoxylin content of various sapanwood and the relative contents of haematoxylin in an oxidized state and a reduced state can be better measured.

Description

technical field [0001] The invention relates to a method for electrochemically measuring hematoxylin content in hematoxylin. Background technique [0002] Because plant polyphenols coexist and are similar to flavonoids, anthraquinones, simple phenols and lignin in plants. It itself is a kind of mixture with very similar structure and properties, and polyphenols are more aggressive and prone to condensation or degradation, so it is difficult to accurately quantify them. Although there are dozens of polyphenol quantification methods, these methods are relative, and almost none of them can be applied to all determinations. The determination method and related test conditions must be selected according to the actual situation. The quantitative methods of plant polyphenols mainly include chemical analysis, ultraviolet visible spectrophotometry (UV) and high performance chromatography (HPLC). Among them, the UV method is more commonly used. Folin-phenol method (FD method) in al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
Inventor 高海燕仇小飞侯秀良汪茹刘瑛陈新许芳萍
Owner JIANGNAN UNIV
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