Porcine parvnvirus living vaccine and preparation method thereof

A parvovirus, live vaccine technology, applied in the direction of antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem of no effective treatment method for PPV infection, reduce cost burden, high antibody titer, Long shelf life effect

Inactive Publication Date: 2010-06-23
上海佳牧生物制品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no effective treatment for PPV infection. Like many other viral d

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of porcine parvovirus disease live vaccine, the method comprises the following steps:

[0036] (1) Prepare ST cells according to conventional methods, the growth medium is MEM containing 8% newborn bovine serum, and when the cells grow into more than 2 / 3 monolayer, they are inoculated with poison;

[0037] (2) Take the attenuated porcine parvovirus PPV for production S-1 A strain virus (PPV S-1 The attenuated strain obtained by continuous subculture on the pig testicular cell line to the 40th generation or more, and its HA titer ≥ 2 9 , TCID 50 ≥107.0 / ml) was diluted at 0.1%, inoculated with a well-growing ST cell monolayer, adsorbed at 37°C for 1.5 hours, added MEM cell maintenance solution containing 2% newborn bovine serum, and placed at 37°C for static or rotational culture;

[0038] (3) Observing twice a day, recording the cytopathic condition, when more than 75% of the cells appear cytopathic, harvest the cell culture, freeze and thaw 3 ti...

Embodiment 2

[0058] A preparation method of porcine parvovirus disease live vaccine, the method comprises the following steps:

[0059] (1) Prepare ST cells according to conventional methods, the growth medium is MEM containing 5% newborn bovine serum, and inoculate when the cells grow into more than 2 / 3 monolayer;

[0060] (2) Take the attenuated porcine parvovirus PPV for production S-1 A strain virus (PPV S-1 The attenuated strain obtained by continuous subculture on the pig testicular cell line to the 40th generation or more, and its HA titer ≥ 2 9 , TCID 50 ≥10 7.0 / ml) was diluted at 0.1%, inoculated with a well-growing ST cell monolayer, adsorbed at 37°C for 1.5 hours, added MEM cell maintenance solution containing 2% newborn bovine serum, and placed at 37°C for static or rotational culture;

[0061] (3) Observing twice a day, recording the cytopathic condition, when more than 75% of the cells appear cytopathic, harvest the cell culture, freeze and thaw 3 times, and store it bel...

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Abstract

The invention relates to a porcine parvnvirus living vaccine and a preparation method thereof. The living vaccine is prepared by adopting a PPVS-1A strain (the HA valence is not less than 29, and the TCID50 is not less than 107.0/ml) to obtain a cell culture through being inoculated with a swine testis (ST) subculture cell growing well, adding a suitable stabilizing agent and carrying out freezing vacuum drying. The virus content of each first part of the living vaccine is not less than 105.0TCID50. Compared with the prior art, the porcine parvnvirus living vaccine has good immunogenicity, rapid antibody generation after immunization, high titer and long maintenance time of the generated antibody, long retention period and small immunizing dose. When the porcine parvnvirus living vaccine is used to carry out vaccine injection a plurality of weeks before hybridization, the pregnant sow can maintain strong immunizing power in the easy infection period. The porcine parvnvirus living vaccine is the excellent choice for preventing the porcine parvnvirus. The adopted preparation method has reasonable process and lower cost and greatly reduces the cost burden of the livestock breeding.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biological medicines, and relates to a porcine parvovirus vaccine, in particular to a porcine parvovirus live vaccine and a preparation method thereof. Background technique [0002] Porcine parvovirus disease is one of porcine reproductive disorders caused by porcine parvovirus (PPV) infection. PPV infection mainly causes the death of embryos and fetuses. Pregnant sows show abortion, stillbirth, mummies and weak offspring, especially in mummified fetuses. At the same time, PPV can aggravate the multisystemic wasting syndrome of weaned piglets caused by porcine circovirus, and co-infection with other immunosuppressive diseases often brings devastating blows to pig farms. The disease is widely distributed all over the world, causing huge economic losses to the pig industry. [0003] Since Mayr isolated and reported the virus for the first time in 1966, porcine parvovirus has been isolated a...

Claims

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Application Information

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IPC IPC(8): A61K39/125A61P31/20
Inventor 张婉华倪建平叶向阳李春华邹勇潘雪珠徐平曹伟明朱永军
Owner 上海佳牧生物制品有限公司
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