Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof
A oat acid bacteria, biodegradation technology, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of not being degraded, unable to effectively remove estrogen, etc., to achieve the effect of increasing the rate
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[0015] 1. Isolation, screening and preservation of the Acidovorax avenae SS-1 strain provided by the present invention
[0016] The activated sludge in the denitrification and phosphorus removal process system of the sewage treatment plant was collected as a microbial source, and transported back to the laboratory under refrigeration. Prepare an inorganic medium containing 50mg / L estrogen as the sole carbon source, and inoculate it in a 500mL Erlenmeyer flask with 100mL of the above-mentioned inorganic medium according to the inoculum amount of 2.5% activated sludge. The inorganic component contained in the medium is K 2 HPO 4 , 0.1g / L; (NH 4 ) 2 HPO 4 , 0.1g / L; MgSO 4 ·7H 2 O, 0.02g / L; FeCl 3 , 0.01g / L; CaCl 2 2H 2O, 0.1g / L; NaCl, 0.1g / L. Use 2‰ acetic acid to adjust the pH value of the medium to 7.0-7.2, and sterilize it in an autoclave at 121°C for 20 minutes. After the temperature drops to about 30°C, inoculate it under the ultra-clean bench. After sludge inocul...
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