Jar external circulatory packed bed type cell reactor and method for cultivating animal cell

A cell reactor, animal cell technology, applied in animal cells, tissue cell/virus culture devices, biochemical equipment and methods, etc., can solve the problems of low dissolved oxygen level, microbial contamination, low expression, etc. Survival conditions, high degree of imitation of life, and rapid oxygen dissolution

Active Publication Date: 2013-08-28
惠识瑶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing reactor adopts the mechanical stirring method inserted into the tank. The structure of the stirring device and its lubricating substances often bring about the problem of microbial contamination. The incidence of bacteria brought into the stirring and lubrication is high. Cell death, or cell death due to mechanical agitation and shearing, and high-density culture, the dissolved oxygen level is often low, which cannot meet the needs, which is also an important reason for low density and low expression

Method used

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  • Jar external circulatory packed bed type cell reactor and method for cultivating animal cell
  • Jar external circulatory packed bed type cell reactor and method for cultivating animal cell
  • Jar external circulatory packed bed type cell reactor and method for cultivating animal cell

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The packed-bed cell reactor with external circulation of the tank comprises: a reaction tank body 100 connected to a shaker 50, and shaking of the shaker makes the reaction tank rotate eccentrically. The upper part of the reaction tank body 100 is in the shape of a truncated cone 107, the lower part is in the shape of a cylinder 109, and the bottom is in the shape of a cone 120. The upper part of the described truncated cone is provided with an outer conduit inlet 105, and the outer conduit is unscrewed around the tank outside the tank. And return to the tank at the bottom, the outer conduit is around the tank body 1800 outside the tank, and the liquid return port 106 of the outer conduit enters the reaction tank at the center of the bottom of the tank, and the reaction The upper cover of the tank body has a tank cover with an air inlet pipe 85, a liquid outlet pipe 86, a liquid inlet pipe 87, a dissolved oxygen probe 80, and a pH probe 81 pass through the tank cover and...

Embodiment 2

[0023] The packed bed type cell reactor with circulation outside the tank of Example 1, the packed bed includes an upper orifice plate 110 , a lower orifice plate 112 , an empty casing 115 and a sheet microcarrier 111 . The cylindrical 109 inner wall of the reaction tank body 100 is connected with the lower orifice 112 and the empty casing 115, the upper end of the empty casing 115 is connected with the upper orifice 110, the upper orifice 110, the empty casing 115 , The lower orifice plate 112 constitutes a packed bed, and the sheet microcarrier 111 is filled between the lower orifice plate and the upper orifice plate. The packed bed is consistent with the center line of the reaction tank body 100 . The reaction tank has a chassis 102, the reaction tank 110 is connected to the shaking plate 50 through the chassis 102, the reaction tank body 100 is equipped with a top cover 101, and the reaction tank body 100 is connected to the upper The covers are connected with bolts.

[...

Embodiment 3

[0026] In the packed bed cell reactor with external circulation of the tank described in embodiment 1 or 2, the semi-vertical angle α of the surface of the upper frustum of the reaction tank body is 25° to 55°, such as 30° or 35°, 40°, 45° or 50° can be determined through experiments according to the dissolved oxygen level of the required nutrient solution and the required circulation speed. Generally, it is better between 30-42°. The outside of the reaction tank has Thermal insulation water jacket 104.

[0027] The packed bed cell reactor with external circulation of the tank, the tank body of the reaction tank and the shaker are fixed with bolts; the tank body and the upper cover are sealed with an "O" rubber ring; The cylinder at the lower part of the reaction tank has rounded corners and transitions to a conical bottom, and the inner bottom of the reaction tank has a conical shape, which is connected to the lower end of the outer conduit through a round sky structure.

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Abstract

The invention relates to a jar external circulatory packed bed type cell reactor and a method for cultivating an animal cell. Following the fast development of the present world biotechnology pharmaceuticals industry which obtains a tremendous achievement, the traditional reactor uses a mechanical stirring mode by inserting in the jar, so the stirring device structure and the lubrication matter thereof often bring microbial contamination or the cell is killed by the mechanical stirring, and the high-density cultivation has a lower dissolved oxygen level which does not meet the demand. The product comprises a reaction jar (100), wherein the bottom of the reaction jar is connected to a shaker (50); the upper part of the reaction jar is provided with an external conduit (105); the external conduit is screwed downwards outside the jar and turns back to the jar from the bottom; a round inner wall of the reaction jar is provided with an inlet of the external conduit; the reaction jar is provided with a jar cover having an air inlet pipe and an air outlet pipe (85); and the interior of the reaction jar is provided with a packed bet having a filling platy micro-carrier (111). The invention provides an excellent environment for cultivating the animal cell.

Description

Technical field: [0001] The invention relates to a cell bioreactor, in particular to a packed-bed cell reactor without a mechanical stirring device for external circulation of a tank for animal cell culture and a method for cultivating animal cells. Background technique: [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, the cell bioreactor used for large-scale high-density cultivation of animal cells has become the most important equipment in the pharmaceutical industry of acoustic biotechnology. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. The existing reactor adopts the mec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/04C12N5/07
Inventor 惠识瑶
Owner 惠识瑶
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