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Fluidized bed type cell reactor circulating outside tank and method for cultivating animal cells

A technology of cell reactors and animal cells, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as low dissolved oxygen level, microbial contamination, cell death, etc., and achieve dissolved oxygen Fast speed, high degree of imitation of life, and the effect of reducing the probability of pollution

Active Publication Date: 2013-09-04
惠识瑶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing reactor adopts the mechanical stirring process inserted into the tank. The structure of the stirring device and its lubricating substances often bring about the problem of microbial contamination. The incidence of bacteria brought into the stirring and lubrication is high. Cell death, or cell death due to mechanical agitation and shearing, and large-scale high-density culture often have low dissolved oxygen levels, which cannot meet the needs, and are also important reasons for low density and low expression

Method used

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  • Fluidized bed type cell reactor circulating outside tank and method for cultivating animal cells
  • Fluidized bed type cell reactor circulating outside tank and method for cultivating animal cells
  • Fluidized bed type cell reactor circulating outside tank and method for cultivating animal cells

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Effect test

Embodiment 1

[0020] The fluidized bed cell reactor with external circulation of the tank comprises: a reaction tank body 100, the bottom of which is connected to a shaking table 50, and the shaking of the shaking table makes the reaction tank body rotate eccentrically. The upper part of the reaction tank body 100 is in the shape of a truncated cone 107, the lower part is in the shape of a cylinder 109, and the bottom is in the shape of a cone 120. The upper part of the truncated cone is equipped with an outer conduit 108, and the entrance of the outer conduit is opened on the On the inner wall of the conical part of the reaction tank, the outer conduit is unscrewed around the tank body outside the tank, and the outer conduit is wound around the tank body 180 outside the tank 0 , the liquid return port 106 at the outlet end of the outer conduit enters the reaction tank body from the center of the bottom of the tank body, the tank body of the reaction tank has a tank cover with an inlet pipe ...

Embodiment 2

[0022] The fluidized bed cell reactor of embodiment 1, which belongs to the external circulation of the tank, has a microporous plate 110, an empty casing 116 and a macroporous microcarrier 111 in the described cell survival area, and the inner cylindrical shape of the reaction tank body 100 is 109 The inner wall and the frustum-shaped 107 inner wall are connected to the upper conical filter screen, and the lower end is connected to the microporous plate 110. The microporous plate 110 is connected to the empty sleeve 116 to form a cell survival area, and the cell survival area is connected to the center line of the reaction tank body. Concentric, the conical 120 lower end of the bottom of the reaction tank is connected to the liquid return port 116 at the end of the outer conduit, so that the communication with the outer conduit is realized at the bottom of the tank; the reaction tank body 100 has a chassis 102, the reaction tank 100 and the shaking plate 50 are connected throu...

Embodiment 3

[0024] The fluidized-bed cell reactor of embodiment 1 or 2 described in the external circulation of the tank, the semi-vertical angle α of the surface of the upper frustum of the tank body of the reaction tank is 25° to 55°, such as 30° or 35° , 40°, 45° or 50°, which can be determined through experiments according to the dissolved oxygen level of the required nutrient solution and the required circulation speed. Generally, it is better between 30-42°. The outside of the reaction tank Thermal insulation water jacket 104 is arranged.

[0025] The fluidized bed cell reactor with external circulation of the tank, the tank body of the reaction tank and the shaker are fixed with bolts; the tank body and the upper cover are sealed with an "O" rubber ring The cylindrical shape of the lower part of the reaction tank body has rounded corners and transitions to the bottom conical shape, and the bottom of the inner side of the reaction tank body has a conical shape. end.

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Abstract

The invention relates to a fluidized bed type cell reactor circulating outside a tank and a method for cultivating animal cells. The traditional reactor adopts the stirring process of a machine inserted in a tank body, a stirring device structure and lubrication matter thereof frequently resulting in the problem of microbial contamination, the condition that stirring and lubrication carry in bacteria has high incidence, and the shearing of mechanical stirring results in cell death. The fluidized bed type cell reactor comprises a reaction tank body (100), wherein the bottom of the reaction tank body is connected with a swing bed (50) which swings to cause the reaction tank body to eccentrically rotate, the upper part of the reaction tank body is provided with an outer conduit (108), an inlet of the outer conduit is arranged on the inner wall of the cone part of the reaction tank body, the outer conduit is screwed off around the tank body along the outer part of the tank body, a liquid return port (106) at the outlet end of the outer conduit enters the reaction tank body from the center of the bottom of the tank body, a tank cover with an inlet tube and an outlet tube is arranged on the reaction tank body in which a cell living zone is arranged, and a conical filter screen (112) is arranged above the cell living zone. The invention provides high-quality environment for the cultivation of the animal cells.

Description

Technical field: [0001] The invention relates to a cell bioreactor, in particular to a fluidized bed cell reactor used for animal cell culture for external circulation of tanks and a related method for culturing animal cells. Background technique: [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, cell bioreactors used for large-scale high-density cultivation of animal cells have become the most important equipment in the biotechnology pharmaceutical industry. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. The existing reactor adopts the mechanical stirring process inserted into...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 惠识瑶
Owner 惠识瑶
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