Marker for detection of IL-17-producing helper T-cell, and method for detection of IL-17-producing helper T-cell

A cell and labeling technology, applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., can solve problems such as unclear reasons

Inactive Publication Date: 2011-01-26
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although RA is an autoimmune disease, its cause has not yet been clarified

Method used

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  • Marker for detection of IL-17-producing helper T-cell, and method for detection of IL-17-producing helper T-cell
  • Marker for detection of IL-17-producing helper T-cell, and method for detection of IL-17-producing helper T-cell
  • Marker for detection of IL-17-producing helper T-cell, and method for detection of IL-17-producing helper T-cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1: Analysis of highly expressed genes in cultured Th17 cells derived from mice

[0107] 1. Isolation of Naive T Cells from Mouse Spleen

[0108] The spleen of the BALB / c mouse was removed to obtain a sample containing spleen cells. After the erythrocytes in the sample were hemolyzed with ammonium chloride, CD8, B cells, monocytes, macrophages, granulocytes, and immature cells were removed from the sample with magnetic beads (manufactured by POLYSCIENCE). Cellular components of red blood cells, crude CD4 positive (CD4 + ) T cells. CD4 + T cells were sorted by flow cytometry, and fractions of naive T cells (CD4 + / CD25neg / CD44low / CD62high). Likewise, naive T cells were purified from splenocytes of C57 / BL6 mice.

[0109] 2. Differentiate and culture Th1, Th2, Treg, Th17 cells from naive T cells

[0110]The naive T cells derived from BALB / c mice obtained in the above 1. were mixed with 0.5-2.0×10 6 Cells / 2ml / well were seeded onto 24-well plates containing an...

Embodiment 2

[0143] Example 2: Analysis of highly expressed genes in disease model mice

[0144] 1. Making disease model mice

[0145] 1) Making SKG arthritis model mice (hereinafter referred to as "arthritis model mice")

[0146] Arthritis model mice were produced by the following method

[0147] a) Preparation of bacteria components and administration to mice

[0148] Suspend the thermocurdlan (curdlan) (SIGMA company) produced by Alcaligenes faecalis in PBS (phosphate buffered saline) to obtain thermocurdlan preparation (50mg / ml) (hereinafter referred to as thalline Element). The bacterium component was administered to the abdominal cavity of 7-8 week-old spontaneous SKG arthritis mice (female) (Nature, vol 426, pp.454-460 (2003), purchased from clea-japan company) 200 μl per mouse . Four weeks later, 200 μl was intraperitoneally administered to each mouse.

[0149] b) Judging the severity of arthritis

[0150] 0 points: normal

[0151] 1 point: Mild joint inflammation

[0152]...

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PUM

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Abstract

Disclosed is a polynucleotide marker or a protein marker for use in the specific detection of an IL-17-producing helper T-cell (a Th17 cell). Also disclosed is a method for detecting a Th17 cell, which is characterized by detecting the occurrence of the polynucleotide marker or the protein marker.

Description

Technical field: [0001] The present invention relates to a marker for detecting helper T cells producing interleukin (IL)-17 (hereinafter referred to as "Th17 cells") and a method for detecting Th17 cells. Background technique: [0002] Rheumatoid arthritis (hereinafter referred to as "RA") is a systemic inflammatory autoimmune disease with arthritis as the main clinical symptom. Although RA can be judged by visual means such as joint pain and other subjective symptoms and swelling degree and observation of bone X-ray, a quantitative index has not yet been established. Therefore, there is currently no established quantitative method that can continuously monitor the effect of treatment. [0003] Although RA is an autoimmune disease, its cause has not yet been clarified. It is generally believed that bacterial infection and the like are the triggers, which cause joint tissue inflammation through a complex network of immune cell groups and cytokine groups. [0004] Central ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/09C12Q1/04G01N33/48G01N33/53
CPCC12Q1/6883G01N33/56972G01N2800/102G01N2333/54C12Q1/6881G01N33/6869C12Q2600/158
Inventor 池田昌郁宇贺均田中聪宫本佳昭柳田匡俊仓田宽一门脇正和
Owner SYSMEX CORP
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