Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.2 with high affinity

A technology of monoclonal antibody, fmu-seb-no.2, which is applied in the field of biomedicine to achieve strong neutralizing ability and high affinity

Active Publication Date: 2012-08-01
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The U.S. military’s 10-year toxin warfare agent prevention and control research plan has always regarded SEB as one of the important research objects, focusing on SEB damage therapeutic antibodies and small molecule inhibitors, but there are only reports on the preparation of polyclonal antibodies (PcAbs). Sexual monoclonal antibodies (mAbs) are still in their infancy

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  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.2 with high affinity
  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.2 with high affinity
  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.2 with high affinity

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Embodiment Construction

[0030] The present invention immunizes Balb / c mice with natural SEB, prepares a group of mouse anti-SEB monoclonal antibodies, screens out the FMU-SEB-No.2mAb hybridoma cell line that can stably secrete high-affinity anti-SEB, and prepares ascites Obtain high-affinity anti-SEB mAb; and confirm the uniqueness of the gene sequence and corresponding protein sequence and its CDR sequence; provide support for anti-SEB chimeric or humanized genetically engineered antibodies. The present invention will be described in detail below in conjunction with the preparation method of specific monoclonal antibodies, antibody activity detection, and sequence detection and uniqueness determination, which is an explanation rather than a limitation of the present invention. The present invention is specifically implemented according to the following steps:

[0031] 1. Preparation of mouse anti-SEB high affinity antibody FMU-SEB-No.2mAb

[0032] 1.1 Preparation and purification of monoclonal anti...

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Abstract

The invention discloses light chain and heavy chain variable regions of an anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.2 with high affinity. The amino acid sequence of the FMU-SEB-No.2 mAb light chain variable region is shown in SEQ.ID.NO.1, and the amino acid sequence of the FMU-SEB-No.2 mAb heavy chain variable region is shown in SEQ.ID.NO.2. A method for the invention comprises the following steps of: preparing a group of mouse anti-SEB mAbs by using a natural SEB immune Balb / c mouse, screening hybridoma cell lines which can stably secrete high-affinity anti-SEB mAb; and preparing ascitic fluid to obtain the high-affinity anti-SEB mAb FMU-SEB-No.2. The invention facilitates to confirm the uniqueness of a gene sequence and a corresponding protein sequence as well as a CDR sequence thereof, and provides support for researching and developing an anti-SEB mosaic or humanized genetic engineering antibody.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a monoclonal antibody, in particular to the light chain and heavy chain variable regions of anti-SEB monoclonal antibody FMU-SEB-No.2, including its amino acid sequence and nucleotide sequence. Background technique [0002] Biological threat and its defense is one of the most important national security concerns in the world, and it is also one of the important fields of military medical research. Biological threats mainly include biological warfare and bioterrorism, which refers to the use of biological agents (including warfare agents) to deliberately launch attacks on specific target groups and other organisms (such as animals, plants) and facilities closely related to human production and life. . Therefore, biological threats can not only kill and injure a large number of people and animals, but also cause huge psychological panic, social chaos, and economic losses among th...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K16/12C12N15/11G01N33/577G01N33/569
Inventor金伯泉杨琨孙元杰李永明宋朝君董邦权
OwnerFOURTH MILITARY MEDICAL UNIVERSITY