Medicinal composition for preventing and treating bronchial asthma and preparation and application thereof
A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine and natural medicine, can solve the problems of insignificant clinical efficacy of traditional Chinese medicine, and achieve the effects of reducing inflammatory cell infiltration in lung tissue, reducing smooth muscle hyperplasia, and reducing attack symptoms
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Embodiment 1
[0050] Embodiment 1: the preparation of Chinese medicine extract
[0051]1. Weigh the crude drug according to the formula in Table 1
[0052] 2. Extraction:
[0053] Decoction: After mixing the crude drugs, add 13 times of water for the first time, boil for 1 hour, keep warm for 2 hours, and filter out the drug solution. After adding 11 times of water for the second time and boiling, keep it warm for 1 hour, and filter out the medicinal solution in 2 hours. The filtrates were combined and concentrated.
[0054] Alcohol precipitation: add 95% ethanol in an appropriate amount to reach an alcohol content of 63.3%, and refrigerate for 24 hours. Filter out and concentrate the filtrate to a liquid extract sample.
[0055] Table 1 (in the table, the measurement unit of all materials is g)
[0056] formula
Embodiment 2
[0057] Embodiment 2 pharmacodynamic research
[0058] 1. Preparation of modeling liquid
[0059] Sensitization solution: Dissolve 10g of OVA (ovalbumin) in 100ml of physiological saline to prepare a 10% concentration of sensitization solution, which is prepared before use.
[0060] Atomized stimulation solution: Dissolve 5g of OVA in 100ml of physiological saline to prepare a 5% concentration of the sensitization solution, which is prepared before use.
[0061] 2. Preparation of bronchial asthma model
[0062] Dissolving 10 g of OVA in 100 ml of physiological saline to prepare a sensitization solution with a concentration of 10%, sensitizes the mice by intraperitoneal injection to create a mouse model of bronchial asthma. Except for the blank control group, each mouse was sensitized by intraperitoneal injection of 1ml of 10% OVA saline solution on the 1st day and the 13th day; In a 5L airtight container, inhale 5% OVA saline for 40 minutes with an ultrasonic nebulizer to st...
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