Method for preparing special component of traditional Chinese medicine Indian trumpetflower seed, namely Oroxin B
A technology of cypressoside and cypressoside, applied in the field of separating and purifying the preparation of exclusive component cymoxidin B, can solve the problems of environmental and human health effects, toxic and side effects, etc.
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[0006] Cut the medicinal material of Oryza sativa into pieces, add 8 times the amount of 70% ethanol to heat and reflux for 3 times, filter, concentrate the filtrate and extract with water-saturated n-butanol, combine the n-butanol liquid, and recover the n-butanol to obtain the extract. Then extract the n-butanol to obtain the extract, separated by a polyamide column, first eluted with distilled water until the eluent is colorless, discarded the water, and then eluted with 10%-40% methanol to colorless, collect the eluent It is recovered to a small volume and purified by dextran gel, the main color band is collected, the solvent is recovered, and the precipitate is precipitated and recrystallized to obtain oriloside B with a purity of more than 98%.
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