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Grass carp injection vaccine synergist and preparation method thereof

A technology of a synergist and a vaccine, which is applied to the field of injection vaccine synergist and its preparation, can solve the problems of large side effects in animals, unstable adjuvant effect, etc., and achieves a simple and easy-to-operate preparation method, low cost, and synergistic effect. obvious effect

Inactive Publication Date: 2012-07-11
GUANGDONG HAID GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the prior art such as unstable adjuvant effect of immune injections and large side effects on animals, and provide a vaccine synergist for grass carp injection

Method used

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  • Grass carp injection vaccine synergist and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of synergist

[0023] Medium formula: 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract + 20g agar, dissolved in 1L distilled water and then autoclaved. After the Bacillus subtilis was cultured in the above liquid medium for 12 hours, it was washed 3 times with 0.01 M PBS, pH 7.2 phosphate buffer, and then adjusted to 3×10 with PBS. 10 CFU / mL, stored at 4°C. Weigh the yeast β-1,3 glucan and galactomannan oligosaccharide according to the volume of the bacterial liquid before injection, so that the final concentrations in the synergist are 0.2 g / mL and 0.1 g / mL respectively, and the synergist Add it to the vaccine at a ratio of 1:50 and prepare it for immediate use.

Embodiment 2

[0024] Embodiment 2: the preparation of synergist

[0025] Medium formula: 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract + 20g agar, dissolved in 1L distilled water and then autoclaved. After the Bacillus subtilis was cultured in the above liquid medium for 12 hours, it was washed 3 times with 0.01 M PBS, pH 7.2 phosphate buffer, and then adjusted to 3×10 with PBS. 10 CFU / mL, stored at 4°C. Weigh the yeast β-1,3 glucan and galactomannan oligosaccharide according to the volume of the bacterial liquid before injection, so that the final concentrations in the synergist are 0.2 g / mL and 0.1 g / mL respectively, and the synergist Add it to the vaccine at a ratio of 1:100 and prepare it for immediate use.

Embodiment 3

[0026] Embodiment 3: the synergistic effect of synergist of the present invention to grass carp attenuated vaccine

[0027] Select the synergist in embodiment 1 and 2 to synergize grass carp attenuated vaccine. Grass carp attenuated vaccine is the attenuated grass carp freeze-dried cell vaccine produced by Pearl River Fisheries Research Institute. A total of 600 grass carp species with healthy physique, weighing 50±5g, were selected and stocked in three 3m×2m×2m cages with a water temperature of 28±2°C. 0.2 mL / tail was injected at the base of the dorsal fin of the effective group, normal vaccine group, and control group (PBS). After 28 days of immunization, 30 test fish in the cage were randomly selected and injected with 0.3 mL of 1:10 virus tissue slurry for challenge. After 14 days of observation, the mortality rate was counted, and the survival rate and relative immune protection rate were calculated according to the following formula. The experimental results are shown i...

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Abstract

The invention discloses a grass carp injection vaccine synergist. One milliliter of the synergist comprises the following effective components: 0.1-0.2g of yeast beta-1,3glucan, 0.05-0.1g of galacto-mannan-oligoscessharides and 3*108-3*1010CFU of bacillus subtilis. The invention also discloses a preparation method of the grass carp injection vaccine synergist, which comprises the steps of: adding1-2g of yeast beta-1,3glucan and 0.5-1g of galacto-mannan-oligoscessharides in 10 ml of bacillus subtilis liquid and mixing uniformly. The invention also discloses the application of the grass carp injection vaccine synergist to the vaccination of grass crap. Compared with the prior art, the grass carp injection vaccine synergist has less components, is prepared with a simple method, has obvious synergy on grass carp vaccines, and can improve the protection effect of vaccines on grass carp and prolong immunity protection period.

Description

technical field [0001] The invention relates to an injectable vaccine synergist for grass carp and a preparation method thereof. Background technique [0002] At present, the problem of grass carp disease is still one of the important factors that determine the success of farming. Although traditional chemical drug control can play a certain role, it also causes problems such as pathogen resistance, pathogen mutation frequency, and breeding environment imbalance, which seriously threatens human health and is not conducive to the sustainable development of aquaculture. Immunization control is to improve the specific antibody level of aquatic animals to pathogens through immunization, so as to reduce the occurrence of diseases. Immunization prevention and treatment has no toxic side effects, is green and environmentally friendly, and can fundamentally improve the body's resistance. It is an inevitable trend in the prevention and treatment of aquatic animal diseases in the fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K36/064A23K1/16A61P37/04A61K31/715A61K31/716A61K35/74A23K10/18A23K20/163A23K50/80A61K35/742
Inventor 余露军
Owner GUANGDONG HAID GROUP
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