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Method for quantitatively measuring baicalein and wogonin in scutellaria baicalensis simultaneously

A technology of wogonin and its determination method, which is applied in the field of quality control of traditional Chinese medicine, can solve the problems such as not finding one article, and achieve the effects of good peak separation, complete enzymatic hydrolysis, and simple method

Inactive Publication Date: 2011-07-20
北京赛尔泰和生物医药科技有限公司
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Problems solved by technology

However, there is no report on how much hydrolyzable baicalein and other aglycones are contained in the medicinal materials of Scutellaria baicalensis. The content of baicalein in the medicinal materials of Scutellaria baicalensis is calculated from the content of baicalin according to the molecular weight. Whether the calculated theoretical value is consistent with the real content is unknown

Method used

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  • Method for quantitatively measuring baicalein and wogonin in scutellaria baicalensis simultaneously
  • Method for quantitatively measuring baicalein and wogonin in scutellaria baicalensis simultaneously
  • Method for quantitatively measuring baicalein and wogonin in scutellaria baicalensis simultaneously

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Experimental program
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Effect test

Embodiment 1

[0071] 1. Chromatographic conditions: chromatographic column: octadecylsilane bonded silica gel as filler; mobile phase: methanol-0.1% phosphoric acid solution, volume ratio is 51:49; detection wavelength: 275nm; column temperature: room temperature; flow rate: 1.0ml / min; number of theoretical plates: calculated based on baicalein peak, should not be less than 3000.

[0072] 2. Preparation of reference substance solution Accurately weigh the appropriate amount of baicalein and wogonin reference substances into a brown measuring bottle, add methanol respectively to make a solution containing 30 μg of baicalein and 8 μg of wogonin per 1 ml.

[0073] 3. Preparation of the test solution Take 0.02g of Scutellaria baicalensis powder, weigh it accurately, place it in a 50ml measuring bottle, add 8ml of 35°C water, and enzymolyze it in a water bath at the same temperature for 2 hours, take it out, add the volume Take 35ml of methanol-hydrochloric acid mixture with a ratio of 200:1, so...

Embodiment 2

[0076] 1. Chromatographic conditions: chromatographic column: octadecylsilane bonded silica gel as filler; mobile phase: methanol-0.1% phosphoric acid solution, volume ratio is 52:48; detection wavelength: 275nm; column temperature: room temperature; flow rate: 1.0ml / min; number of theoretical plates: calculated based on baicalein peak, should not be less than 3000.

[0077] 2. Preparation of reference substance solution Accurately weigh the appropriate amount of baicalein and wogonin reference substances into a brown measuring bottle, add methanol respectively to make a solution containing 33 g of baicalein and 9 μg of wogonin per 1 ml, and obtain the solution.

[0078] 3. Preparation of the test solution Take 0.02g of Scutellaria baicalensis powder, weigh it accurately, place it in a 50ml measuring bottle, add 5ml of 45°C water, and enzymolyze it in a water bath at the same temperature for 2 hours, take it out, add volume Take 37ml of methanol-hydrochloric acid mixture with ...

Embodiment 3

[0081] 1. Chromatographic conditions: chromatographic column: octadecylsilane bonded silica gel as filler; mobile phase: methanol-0.1% phosphoric acid solution, volume ratio is 53:47; detection wavelength: 275nm; column temperature: room temperature; flow rate: 1.0ml / min; number of theoretical plates: calculated based on baicalein peak, should not be less than 3000.

[0082] 2. Preparation of reference substance solution Precisely weigh the appropriate amount of baicalein and wogonin reference substances into a brown measuring bottle, add methanol respectively to make a solution containing 35 μg of baicalein and 10 μg of wogonin per 1 ml.

[0083] 3. Preparation of the test solution Take 0.02g of Scutellaria baicalensis powder, weigh it accurately, place it in a 50ml measuring bottle, add 6ml of 50°C water, and enzymolyze it in a water bath at the same temperature for 2.5 hours, take it out, add volume Take 36ml of methanol-hydrochloric acid mixture with a ratio of 200:1, soni...

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Abstract

The invention discloses a self enzymolysis approach method of baicalein and wogonin in scutellaria baicalensis. The measurement method disclosed by the invention comprises the following steps of: carrying out enzymolysis on saponin compounds, such as baicalin, wogonoside, and the like in an aqueous solution by utilizing enzyme contained in scutellaria baicalensis at a proper temperature; after finishing enzymolysis, adding acid methanol; maintaining the stability of the baicalein when killing scutellaria baicalensis enzyme; fully dissolving emzymatically-decomposed baicalein; and filtering and adding filtered liquid as a sample solution. The baicalein and wogonin in the scutellaria baicalensis are simultaneously measured by an HPLC (High Performance Liquid Chromatography) by using a methanol-0.1 percent phosphoric acid solution with the volume ratio of (51-54):(49-46) as a mobile phase and 275nm as a detecting wavelength. The method has the advantages of simplicity, convenience, high speed, complete enzymolysis of components to be detected, and favorable wave crest separation. The self enzymolysis approach method of baicalein and wogonin in scutellaria baicalensis is reported for the first time and provides real basic content data for the full extraction and separation of the components. A content practical measured value of the baicalein is larger than a theoretical value calculated by molecular weights of baicalin and baicalein.

Description

technical field [0001] The invention relates to a quality control method of Scutellaria baicalensis, in particular to a simultaneous quantitative determination method for baicalein and wogonin in Scutellaria baicalensis, belonging to the field of quality control of traditional Chinese medicines. Background technique [0002] Scutellaria baicalensis is a commonly used traditional Chinese medicine. It is recorded in the calendar editions of the Chinese Pharmacopoeia. For damp-warm, summer-warm, chest tightness, nausea, damp-heat fullness, dysentery, jaundice, lung-heat, cough, high fever, dysphoric cough, blood-heat vomiting at the nose, carbuncle sores, restless fetal movement. The active ingredients in Scutellaria baicalensis mainly include flavonoids such as baicalin, wogonoside and their aglycones. Among them, the content of baicalin is the highest, which can reach 18%. According to the item of Scutellaria baicalensis in Chinese Pharmacopoeia, it is stipulated that baical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06A61K36/539
Inventor 韩桂茹李晓燕赵韶华
Owner 北京赛尔泰和生物医药科技有限公司
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