Method for quantitatively measuring baicalein and wogonin in scutellaria baicalensis simultaneously

A technology for wogonin and baicalein, which is applied in the field of quality control of traditional Chinese medicines, can solve problems such as one that has not been consulted, and achieve the effects of good peak separation, complete enzymatic hydrolysis, and simple method.
CN102128889BInactive Publication Date: 2013-05-08北京赛尔泰和生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京赛尔泰和生物医药科技有限公司
Publication Date
2013-05-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a self enzymolysis approach method of baicalein and wogonin in scutellaria baicalensis. The measurement method disclosed by the invention comprises the following steps of: carrying out enzymolysis on saponin compounds, such as baicalin, wogonoside, and the like in an aqueous solution by utilizing enzyme contained in scutellaria baicalensis at a proper temperature; after finishing enzymolysis, adding acid methanol; maintaining the stability of the baicalein when killing scutellaria baicalensis enzyme; fully dissolving emzymatically-decomposed baicalein; and filtering and adding filtered liquid as a sample solution. The baicalein and wogonin in the scutellaria baicalensis are simultaneously measured by an HPLC (High Performance Liquid Chromatography) by using a methanol-0.1 percent phosphoric acid solution with the volume ratio of (51-54):(49-46) as a mobile phase and 275nm as a detecting wavelength. The method has the advantages of simplicity, convenience, high speed, complete enzymolysis of components to be detected, and favorable wave crest separation. The self enzymolysis approach method of baicalein and wogonin in scutellaria baicalensis is reported for the first time and provides real basic content data for the full extraction and separation of the components. A content practical measured value of the baicalein is larger than a theoretical value calculated by molecular weights of baicalin and baicalein.
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Description

technical field

[0001] The invention relates to a method for quality control of Scutellaria baicalensis, in particular to a method for measuring baicalein and wogonin in Scutellaria baicalensis by self-enzymatic hydrolysis, belonging to the field of quality control of traditional Chinese medicines. Background technique

[0002] Scutellaria baicalensis is a commonly used traditional Chinese medicine. It is recorded in the calendar editions of the Chinese Pharmacopoeia. For damp-warm, summer-warm, chest tightness, nausea, damp-heat fullness, dysentery, jaundice, lung-heat, cough, high fever, dysphoric cough, blood-heat vomiting at the nose, carbuncle sores, restless fetal movement. The active ingredients in Scutellaria baicalensis mainly include flavonoids such as baicalin, wogonoside and their aglycones. Among them, the content of baicalin is the highest, which can reach 18%. According to the item of Scutellaria baicalensis in Chinese Pharmacopoeia, it is stipulated that bai...

Claims

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