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Application of biocatalysis bacterial strain in converting dextro-fosfomycin into levorotatory fosfomycin

A technology of D-fosfomycin and L-fosfomycin, which is applied in the application field of biocatalytic strains in converting D-fosfomycin into L-fosfomycin, and can solve problems such as environmental pollution and waste of resources

Active Publication Date: 2013-03-20
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1
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Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of application of biocatalytic bacterial strain in converting D-fosfomycin into L-fosfomycin, and provide technology for solving the serious waste of resources and environmental pollution of D-fosfomycin in the prior art Base

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  • Application of biocatalysis bacterial strain in converting dextro-fosfomycin into levorotatory fosfomycin

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Embodiment

[0025] First three bacterial strains, namely: Citrobacter freundii (Citrobacter freundii, ATCC 8090), Pseudomonas sp. ATCC 11922, and Clostridium sporogenes (ATCC 3584), were cultured separately Base enrichment culture, centrifuge, collect bacteria. The components of the complete medium are: by weight percentage, beef extract 0.5%, peptone 1%, NaCl 0.2%, agar 2%, the balance is water; pH 7.5, 121°C damp heat sterilization for 20min. The enrichment culture condition is a constant temperature shaking culture, a culture temperature of 30° C., a shaker rotation speed of 150 rpm, and culture for 24 hours.

[0026]The collected thalline is inoculated in the catalytic medium again, the inoculum of Citrobacter freundii, Pseudomonas and Clostridium account for 6%, 2%, 4% of the weight of the catalytic medium respectively, at a constant temperature of 37°C The culture was shaken for 96 hours, and the fermentation broth was further tested to see if it contained levofosfomycin. Catalyti...

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Abstract

The invention relates to the fields of biomedicine and biotechnology, and in particular relates to an application of a biocatalysis bacterial strain in converting dextro-fosfomycin into levorotatory fosfomycin. Three bacteria bacterial strains can be used for converting the dextro-fosfomycin into the levorotatory fosfomycin by biocatalysis, thereby providing the technical basis for solving the existing problems of severe resource waste, environmental pollution and the like of the dextro-fosfomycin. The dextro-fosfomycin is a by-product formed in the chemical synthesis process of the levorotatory fosfomycin, has no drug effect, is abandoned as a waste, pollutes environment and wastes resource. The application proves that the mixed culture of the three bacteria bacterial strains has the capability of converting the dextro-fosfomycin into the levorotatory fosfomycin by biocatalysis. The three bacteria bacterial strains are respectively Citrobacter freundii, pseudomonas and Clostridium sporogenes. The three bacteria bacterial strains provided by the invention have catalytic capability and have the possibility of changing the existing abandoned dextro-fosfomycin into valuable things and possibility of being reused by the biocatalysis technology.

Description

technical field [0001] The invention relates to the fields of biomedicine and biotechnology, in particular to the application of a biocatalyzed bacterial strain in converting D-fosfomycin into L-fosfomycin. Background technique [0002] Levofosfomycin [(-)-cis-(1R,2S)-1,2-epoxypropyl phosphate, FOM] is a broad-spectrum antibiotic. Unlike other antibiotics, fosfomycin has a unique chemical structure and antibacterial mechanism. There is no cross-resistance between fosfomycin and other antibiotics, and synergistic effect can be presented. At the same time, because fosfomycin also has the advantages of stable physical and chemical properties, safety, low toxicity, and no antigenicity, it has been widely used in clinical treatment and is called a new antibiotic in the 21st century. [0003] At present, the production process of fosfomycin adopts chemical synthesis technology, and the yield of this technology is low. The main reason is that the epoxidation step in the process i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P17/02C12R1/01C12R1/38C12R1/145
Inventor 徐慧王若超杨伟超周丽沙韩斯琴孔双纪悦周凯赵华季光辉金玉坤
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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