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Method for conversion from right-handed phosphonomycin to left-handed phosphonomycin

A technology of levofosfomycin and dextrofosfomycin, applied in the field of biomedicine

Active Publication Date: 2008-04-02
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention proposes a biocatalytic method for converting D-fosfomycin into L-fosfomycin to solve the problem of converting D-fosfomycin into L-fosfomycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Catalytic strains are used to combine bacterial strain No.1, which contains three kinds of bacteria—Acinetobacter ruckeri, Arthrobacter simplex, and Radiation-resistant Rhodobacter. These 3 kinds of bacterial strains were amplified and cultivated respectively, and the amplified culture medium adopted was as follows: by weight percentage, beef extract 0.5%, peptone 1%, NaCl 0.2%, agar 2%, the balance was tap water, pH 7.5. 121 ℃ damp heat Bacteria 20min. Culture conditions: temperature 28° C., 200 rpm, culture for 5 days. After centrifuging and collecting the bacteria, they were inoculated in the catalytic medium at the same time. The three catalytic bacterial strains were added to the catalytic medium in the form of bacterial suspensions. 3%; wherein, the catalytic bacterial strain bacterial suspension is that the catalytic bacterial strain is added to normal saline, and the concentration of the catalytic bacterial strain is 10% 7 cells / ml of bacterial suspension. Ca...

Embodiment 2

[0025] Catalyst strains are used to combine strain No.3, which contains five kinds of bacteria—Acinetobacter ruckeri, Arthrobacter simplex, Radiation-resistant Rhodobacter, Chryseobacterium acetrichum and Pseudomonas putida. The five kinds of bacteria were amplified and cultivated respectively, and the amplified culture medium adopted was as follows: by weight percentage, beef extract 0.5%, peptone 1%, NaCl 0.2%, agar 2%, the balance was tap water, pH 7.5. 121 ℃ damp heat Bacteria 20min. Culture conditions: temperature 30° C., 150 rpm, culture for 4 days. After centrifuging and collecting the bacteria, they were inoculated in the catalytic medium at the same time. The five catalytic bacterial strains were added to the catalytic medium in the form of bacterial suspensions. 2%; wherein, the catalytic bacterial strain bacterial suspension is that the catalytic bacterial strain is added to normal saline, and the concentration of the catalytic bacterial strain is 10% 8 cells / ml o...

Embodiment 3

[0027] Adopt a kind of catalytic bacterial strain-irradiation resistant red bacillus, its amplified culture, the amplified culture medium that adopts is as follows: by weight percentage, beef extract 0.5%, peptone 1%, NaCl 0.2%, agar 2%, balance is water , pH 7.5. Sterilize with damp heat at 121°C for 20min. Culture conditions: temperature 28° C., 180 rpm, culture for 7 days. After centrifuging and collecting the bacteria, they are inoculated in the catalytic medium at the same time, and the catalytic bacterial strain is added to the catalytic medium in the form of a bacterial suspension, and the amount of the bacterial suspension accounts for 20% of the weight of the catalytic medium; wherein, the catalytic bacterial strain The bacterial suspension was added to the physiological saline as the catalytic bacterial strain, and the concentration of the catalytic bacterial strain was 10 6 cells / ml of bacterial suspension. Catalytic medium component is: by weight percentage, Defo...

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PUM

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Abstract

The invention relates to the biological medicine and biological catalytic field, in particular to a biological catalytic method for transforming the D-fosfomycin into the L-fosfomycin through a biological catalytic process, aiming to solve the puzzle to transform the D-fosfomycin into the L-fosfomycin. The strain with catalytic ability is proliferated, centrifuged, harvested and inoculated into a cultivation substrate, which comprises the components by weight percentage of: 0.5-3 percent of D-fosfomycin, 0.5-1.0 percent of (NH4)2SO4, 0.2 percent of NaCl, 0.1 percent of KCl, 0.01-0.02 percet of MgSO4.7H2O, 0.01-0.03 percent of FeSO4.7H2O,0.05-0.15 percent of ,KH2PO4; the left volume is water, and the pH is 7.5. The sterilization is carried out at 121 DEG C for 20 minutes, and cultivation is made for 3-7 days under the temperature of 28-37 DEG C, and the rotation velocity of 150-250 rpm of the rocking bed. The qualitative and quantitative analysis on the L-fosfomycin in the fermentation liquid is carried out respectively through thin-layer chromatography and the method of bioassay disc. The invention provides a novel catalytic method for transforming the D-fosfomycin into the L-fosfomycin, and accomplishes the operable bio-catalysis of the D-fosfomycin.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for converting D-fosfomycin into L-fosfomycin through a biocatalytic process of microorganisms. Background technique [0002] Levofosfomycin [(-)-cis-(1R,2S)-1,2-epoxypropyl phosphate, FOM] is a broad-spectrum antibiotic. Unlike other antibiotics, fosfomycin has a unique chemical structure and antibacterial mechanism. There is no cross-resistance between fosfomycin and other antibiotics, and synergistic effect can be exhibited. At the same time, because fosfomycin also has the advantages of stable physical and chemical properties, safety and low toxicity, no antigenicity (no need for allergy testing), etc., it has been widely used in clinical treatment, and is called a new drug in the 21st century by the international medical and pharmaceutical circles. antibiotic. [0003] At present, the production process of fosfomycin adopts chemical synthesis technology, and the yield ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12P39/00C12N1/20C12R1/01C12R1/365C12R1/40
Inventor 徐慧周丽沙张颖李慧李启平季光辉杨伟超史荣久韩斯琴倪志龙
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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