Method for preparing asexual spores of Antrodia camphorata based on submerged fermentation and application thereof
A technology of submerged liquid fermentation and asexual spores of Antrodia camphorata, applied in the field of bioengineering, can solve the problems of long fermentation cycle of Antrodia camphorata, reduce the chance of contamination by bacteria, improve the quality of fermentation products, and reduce costs
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Embodiment 1
[0021] Preparation of Asexual Spores of Antrodia Camphorata by Shaking Flask Fermentation
[0022] 1. Bacterial strain: the strain of Antrodia camphorata (Antrodia camphorata) whose preservation number is ATCC No. 200183 is used as the starting strain.
[0023] 2. Slant culture: transfer the Antrodia camphorata mycelia to the slant medium, and culture at 28°C for 288 hours.
[0024] Incline medium (g / L): potato 200, glucose 20, agar 20, pH natural.
[0025] 3. Seed shake flask culture: transfer the slant strain into a 500 mL shake flask filled with 100 mL shake flask culture medium, cultivate at 26° C., rotate at 100 rpm, and cultivate for 72 hours.
[0026] Shake flask medium (g / L): glucose 20, peptone 2, magnesium sulfate 0.75, potassium dihydrogen phosphate 1.5, bran 2, pH 4.5.
[0027] 4. Fermentation shake flask culture: Antrodia camphorata fermented liquid was transferred into a 500mL fermentation shake flask equipped with 100mL fermentation medium according to the ino...
Embodiment 2
[0031] Preparation of Asexual Spores of Antrodia Camphorata by Fermentation Tank
[0032] 1. Bacterial species: Antrodia camphorata (Antrodia camphorata) strain with preservation number BCRC No. 35398 was used as the starting bacterial species.
[0033] 2. Slant culture: transfer the Antrodia camphorata mycelia to the slant medium, and culture at 26° C. for 312 hours.
[0034] Slant medium (g / L): potato 180, glucose 18, agar 18, natural pH.
[0035] 3. Seed shake flask culture: transfer the slanted strain of Antrodia camphorata into a 500 mL shake flask filled with 100 mL shake flask culture medium, culture at 26°C, rotate at 100 rpm, and cultivate for 48 hours.
[0036] Shake flask medium (g / L): glucose 20, peptone 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 1, bran 2, pH 4.5.
[0037] 4. Fermentation in seed tank: Transfer Antrodia camphorata fermented liquid into a seed tank equipped with seed culture medium according to 15% inoculum amount, cultivate at 26° ...
Embodiment 3
[0043] Fermentation method of Antrodia camphorata using asexual spores as inoculum
[0044] The asexual spores of Antrodia camphorata prepared according to the method described in Example 1 or Example 2 were used as fermented seeds. asexual spores in 1×10 5 The concentration of each / mL is directly inserted into the fermentation medium, and the spores can germinate to form mycelia within 24 hours, and begin to enter the logarithmic phase of growth to grow ( figure 2 ), the biomass reached the highest after 8 days of fermentation. However, it takes 4-5 days to enter the growth logarithmic phase ( figure 2 ), the seed liquid is transferred into the fermentation medium and it takes 12-14 days to complete the fermentation to obtain the fermentation product. Therefore, using asexual spores as the inoculum source of submerged liquid fermentation can shorten the fermentation period of Antrodia camphorata. In addition, the spores are easy to count, which can be beneficial to cont...
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