Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for detecting classical swine fever resistance character of swine and special kit thereof

A swine fever and kit technology, applied in the field of detection of swine fever resistance traits in pigs, can solve problems such as pig production loss, decline in physique and resistance, loss of alleles, etc.

Inactive Publication Date: 2013-02-06
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding goals oriented towards high-lean pigs lead to the loss or frequency reduction of alleles, which leads to the decline of physical fitness and resistance of modern commercial breeds, and with the popularization of intensive feeding methods, pig diseases, especially some recurring Infectious diseases have caused major losses in pig production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting classical swine fever resistance character of swine and special kit thereof
  • Method for detecting classical swine fever resistance character of swine and special kit thereof
  • Method for detecting classical swine fever resistance character of swine and special kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the method for the pig NEAT1 gene RFLP polymorphism detection pig anti-swine fever traits

[0038] 1. Identification of porcine NEAT1 gene RFLP polymorphism site

[0039] Primers were designed according to the sequence of the conserved region of the porcine NEAT1 gene, and the primer sequences were as follows:

[0040] Neat1a: 5'-GGAGGTGGACAAAATTGACC-3' (sequence 3 in the sequence listing),

[0041] Neat1b: 5'-GACACGGGACATTGGAGAAC-3' (SEQ ID NO: 4 in the Sequence Listing).

[0042] The genomic DNA of three Tongcheng pigs, Large White pigs, Landrace pigs, Wuzhishan pigs, Laiwu pigs, and Bamaxiang pigs were used as templates, and Neat1a and Neat1b were used as primers for PCR amplification. The total volume of the PCR reaction was 20 μL. Template DNA is 50ng, containing 1×Buffer, 1.5mmol / L MgCl 2 , the final concentration of dNTP is 200 μmol / L, the final concentration of primers is 0.2 μmol / L, and 1.0 U Taq DNA polymerase (TaKaRa). The PCR amplification ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting a classical swine fever resistance character of a swine and a special kit thereof. A specific primer pair which is provided by the invention for assisting detection of the classical swine fever resistance character of the swine is shown as (a) or (b): (a) the primer pair consisting of a DNA (Deoxyribonucleic Acid) fragment shown as a sequence 5 of a sequence table and a DNA fragment shown as the sequence 6 of the sequence table; and (b) the primer pair consisting of the DNA fragment shown as the sequence 3 of the sequence table and the DNA fragment shown as the sequence 4 of the sequence table. In the method, the primers are designed at a swine NEAT1 homologous sequence conservation region to amplify genome DNA fragments of different types of swine; the obtained fragment comprises a C / G mutation site; and the classical swine fever virus antibody level of the swine is detected by using polymorphism of a BfaI enzyme cutting site caused by the mutation site. Experiments prove that the mutation site is obviously related to the classical swine fever virus antibody level, so that the method for evaluating the classical swine fever virus antibody level by using the mutation site is accurate and feasible.

Description

technical field [0001] The invention relates to a method for detecting swine fever resistance traits of pigs and a special kit thereof. Background technique [0002] Pork is the main source of animal protein food for Chinese residents, and the pig industry plays a pivotal role in the animal husbandry production chain. For decades, modern breeding techniques have significantly improved the production performance of pigs, but mainly for production traits (growth traits and reproductive traits). Compared with production traits, research on pig health and disease resistance is relatively weak. Breeding goals oriented towards high-lean pigs lead to the loss or frequency reduction of alleles, which leads to the decline of physical fitness and resistance of modern commercial breeds, and with the popularization of intensive feeding methods, pig diseases, especially some recurring Infectious diseases have brought significant losses to pig production. Classical swine fever (CSF) is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 敖红张宁波李奎李勇唐中林杨述林储明星
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com