Colloidal gold detecting method and device based on digital image processing

Abstract

The invention relates to a colloidal gold detecting method based on digital image processing, comprising the following steps of: collecting RGB (Red, Green, Blue) coloured images by an area array CCD (Charge Coupled Device) detector, carrying out gray level conversion, edge extraction, background suppression and the like on the collected coloured images, and finally quantitatively calculating thecorresponding concentration result of a sample. The invention also relates to a colloidal gold detecting device based on the digital image processing; and the device can be used for detecting the colloidal gold by the method provided by the invention so that the device not only realizes the negative and positive qualitative judgement of the sample but also realizes the quantitative application onthe lower end.

Description

technical field [0001] The invention relates to the technical field of human body fluid immune detection, in particular to a colloidal gold detection method based on digital image processing and a device thereof. Background technique [0002] Human body fluids contain important information about human health, including the level of various hormones, changes in specific protein concentrations, the presence and amount of pathogens and their corresponding antibodies, etc. Most of these items to be tested can be immuno-infiltrated with colloidal gold Technology (gold standard spot method) to detect, the test result is to observe whether there are red spots on the reaction plate, the depth of the red spots is proportional to the concentration of the analyte, but it can only be qualitatively interpreted by eyes alone, but cannot be quantified. [0003] The gold standard spot method has been widely used in the determination of human chorionic gonadotropin (HCG) in serum or urine. ...

AI Technical Summary

Problems solved by technology

At present, a gold standard spot method quantitative reader (U2 instrument) can measure HCG. Since the detector used in the U2 instrument is a single-point photodiode, it can only mix the signal of the area to be detected in the instrument. ; In addition, the light source it uses is also a single LED, so the light irradiated on the reaction plate is not uniform enough and the luminous intensity is not stable due to the characteristics of the LED itself.

Especially when testing the low-concentration samples of the gold standard spot method, since the U2 instrument can only analyze and calculate the intensity of the photocurrent generated by the photosensitive tube, the U2 instrument has low resolution at the low end or even cannot perform quantitative identification

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