Gene sequence of flavanonol 4-reductase synthesized by anthocyanins of eggplant

A technology of dihydroflavonol and gene sequence, which is applied in the field of gene sequence of eggplant anthocyanin to synthesize dihydroflavonol 4-reductase, and can solve the problems that the same or similar technical subjects have not been found yet

Inactive Publication Date: 2012-10-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After searching the prior art literature, no technical subject identical or similar to the present invention has been found

Method used

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  • Gene sequence of flavanonol 4-reductase synthesized by anthocyanins of eggplant
  • Gene sequence of flavanonol 4-reductase synthesized by anthocyanins of eggplant
  • Gene sequence of flavanonol 4-reductase synthesized by anthocyanins of eggplant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Cloning, expression and identification of dihydroflavonol 4-reductase (YLP SmDFR) from purple eggplant

[0018] Step 1. Cloning of purple eggplant SmDFR dihydroflavonol 4-reductase gene

[0019] 1. Eggplant experiment materials

[0020] Yunnan purple long eggplant (hereinafter referred to as Zichang eggplant, English abbreviated as YLP) was collected and preserved in our laboratory as the experimental material. Zichang eggplant is a representative purple eggplant resource, which is collected from Yuxi City, Yunnan Province. It has the common thornless, purple stem and purple flower phenotypes of purple eggplant cultivars. The fruit at the commercial maturity stage is black and purple, and the fruit is long and large, so it is called Zichang eggplant. The experimental materials were cultivated in artificial plastic greenhouses in the Aerospace Breeding Base of Pujiang Town, Minhang District, Shanghai. Seedlings are raised, grown, and fruited under all natural conditio...

Embodiment 2

[0208] Cloning, expression and identification of dihydroflavonol 4-reductase (YRW SmDFR) from white eggplant

[0209] Step one, the cloning of white eggplant SmDFR dihydroflavonol 4-reductase gene

[0210] 1. Eggplant experiment materials

[0211] Yunnan round white eggplant (hereinafter referred to as round white eggplant, English abbreviation YRW) was collected and preserved in our laboratory as the experimental material. Round white eggplant is a representative white eggplant resource, which is collected from Yuxi City, Yunnan Province. It has the common fruit phenotype of white eggplant cultivars, and also has the phenotypic characteristics of some wild eggplant varieties: spiny, green stems, and white flowers. Phenotype. The fruit at the commercial maturity stage is milky white, large and round, hence the name round white eggplant. The experimental materials were cultivated in artificial plastic greenhouses in the Aerospace Breeding Base of Pujiang Town, Minhang Distri...

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Abstract

The invention relates to a gene sequence of flavanonol 4-reductase synthesized by anthocyanins of an eggplant, belonging to the technical field of biology. The gene sequence has a nucleotide sequence as shown in 1st-1149th places of nucleotide in SEQ ID NO. 1; and another sequence has a nucleotide sequence as shown in 1st-1149th places of the nucleotide in SEQ ID NO. 3. In the invention, an eggplant variety has significant characteristics on the aspects of high anthocyanins, water stress resistance, uvioresistant protection, and the like, can be obtained; substances, receptors, inhibitors or antagonists, and the like relatively interacted with the flavanonol 4-reductase can be screened through various conventional screening methods by utilizing a flavanonol 4-reductase gene of the eggplant.

Description

technical field [0001] The invention relates to a gene sequence in the field of biotechnology, in particular to a gene sequence of eggplant anthocyanin synthesis dihydroflavonol 4-reductase. Background technique [0002] Anthocyanins are the second largest pigment type in plants after chlorophyll in nature, and belong to flavonoids. Anthocyanin is a water-soluble pigment, slightly acidic, and is stored in the vacuoles of plant cells. The colors of many organs and tissues of plants are determined by anthocyanins. In recent decades, the health-care effects of anthocyanins, which are polyphenols, have been deeply studied. The anti-oxidation and free radical scavenging effects of anthocyanins have attracted more and more attention. Eggplant, especially purple eggplant, is one of the few types of vegetables rich in anthocyanins, which is rich in nutritional value. [0003] Davies et al. reviewed in the article "Enhancing anthocyaninproduction by altering competition for substr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02
Inventor 陈火英李翔刘杨王中彦谢立冠
Owner SHANGHAI JIAO TONG UNIV
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