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DNA Molecular Marker Identification Technique to Distinguish Polygonum cephalum and Polygonum nepal

A DNA molecule, Polygonum cephalus technology, applied in the identification of Polygonum cuspidatum and Polygonum nepalensis, in the biological field, can solve the problems of confusing use, difficult to distinguish and identify, etc.

Inactive Publication Date: 2011-12-28
GUIZHOU WARMEN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thereby overcoming the problem in the prior art that the dried medicinal materials of Polygonum cephalum and Polygonum nepalensis are not easy to distinguish and identify, preventing and avoiding the mixed use of raw materials of "Relinqing Granules"

Method used

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  • DNA Molecular Marker Identification Technique to Distinguish Polygonum cephalum and Polygonum nepal
  • DNA Molecular Marker Identification Technique to Distinguish Polygonum cephalum and Polygonum nepal
  • DNA Molecular Marker Identification Technique to Distinguish Polygonum cephalum and Polygonum nepal

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Experimental program
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Embodiment 1

[0147] Example 1: DNA molecular marker identification technology The detection steps for identifying Polygonum cephalum are as follows:

[0148] 1) Synthetic specific identification primers:

[0149] Synthesize a pair of specific identification primers according to the sequence 5'-GCTCGGTTTCAGAATAAGT-3', 5'-TCCCTGAGGTGTTCGTTT-3', and store at 4°C;

[0150] 2) Using the improved CTAB method to extract the DNA gene of Polygonum cephalum and Polygonum nepalei respectively, the specific steps are as follows:

[0151] ①Take 50 mg of Polygonum cephalum and Polygonum nepalensis each, put them into 2 sterilized mortars, add about 2400 μL of CTAB extract respectively, and grind them quickly respectively, add 20 μL of β-mercaptoethanol during the grinding process, and grind to powder Pasty;

[0152] ②Use tweezers to take the centrifuge tubes and place them on the centrifuge plate, number them and set aside;

[0153] ③Use a pipette gun to draw 700 μL of the ground medicinal material e...

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Abstract

The present invention takes Polygonum cephalum, a representative national medicine in Guizhou, as the research object, explores the use of RAPD analysis method to analyze the genetic diversity of Polygonum cephalum and Polygonum nepalensis; and on the basis of RAPD experiments, converts the RAPD method into SCAR markers, It provides an accurate, reliable, rapid and stable method for the identification of Polygonum cephalum and Polygonum nepalensis.

Description

technical field [0001] The invention relates to a method for identifying Polygonum cephalum and Polygonum nepalensis by using DNA molecular marker identification technology, which belongs to the field of biotechnology. Background technique [0002] Polygonum capitatum Buch-Ham.ex D.Don, a plant of the Polygonaceae family, is a commonly used medicinal material for the Miao people in Guizhou. It has a significant effect on the treatment of urinary system diseases; it is the only raw material of the famous Miao medicine "Relinqing Granules". [0003] Polygonum cephalum is a Chinese herbal medicine with high medicinal value, and it is also a representative Miao medicine in Guizhou. The survey results of the medicinal use of Polygonum cephalosporin and its related species show that Polygonum cephalosa and its congeners, Fotanmu, Chishansan, Polygonum deshi, and Polygonum nepal are widely used in Guizhou, and they are all used for rheumatic arthralgia. For the treatment of pain a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 周涛梁斌江维克张丽艳李孟林谢宇王尚华
Owner GUIZHOU WARMEN PHARMA
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