Method for rapid separation and determination of seven volatile phenols in flue gas of main cigarette flow

Abstract

The invention discloses a method for rapid separation and determination of seven volatile phenols in flue gas of main cigarette flow. An ultra-high-performance liquid-phase chromatographic column is adopted, a mobile phase contains beta-CD aqueous solution, acetonitrile and acetic acid, and the seven volatile phenols in the flue gas of the main cigarette flow can be separated and determined rapidly within 9min. The working efficiency can be greatly increased, the costs of analysis and detection can be saved, and the method has a value for large-scale application and promotion.

Description

technical field [0001] The invention relates to a rapid detection method for seven volatile phenols in mainstream smoke of cigarettes. Background technique [0002] Volatile phenol is an important harmful component in mainstream cigarette smoke, which is included in the list of 46 harmful components of the Canadian government and the list of controlled components recommended by the WHO "Tobacco Product Regulation Research Group", so the volatile phenol in cigarette smoke can be accurately determined It is of great significance for the evaluation of the hazards of cigarettes. [0003] Many analytical techniques, usually including complex liquid-liquid extraction, column chromatography and derivatization, have been applied to the analysis of phenols in cigarette smoke, but because cigarette smoke is a very complex mixture, and the content of phenols Therefore, the accurate quantification of phenolic compounds has always been a difficult task. In the early qualitative researc...

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Problems solved by technology

[0004] However, there are two problems in the determination of volatile phenols in cigarette smoke by HPLC: (1) the test time exceeds 20 minutes, which is not conducive to batch sample analysis; (2) due to the isomer m-cresol and p-cresol The structure and physical and chemical properties of the two are very similar, and the current high-efficiency chromatography technology is difficult to separate them, and only the determination results of the mixture of m-cresol and p-cresol can be obtained.

In order to solve the above problems, researchers have tried a variety of methods. The method of rapid separation and determination of volatile phenols established by Chen Zhangyu et al., but the sample pretreatment is cumbersome and time-consuming, and the multi-step transfer operation is easy to cause the loss of the test substance, and the mobile phase uses 2mL / min high flow rate, resulting in too high column pressure, which is detrimental to the service life of the pump and analytical column

Liu Fang and others established a rapid separation column-high performance liquid chromatography method for the determination of phenol, cresol and quinone in mainstream cigarette smoke, and the analysis time is only 6 minutes; but this method cannot separate m-cresol and p-cresol phenol

Recently, Zhang Jie et al [CN 101592638] added β-CD to the mobile phase as a modifier to realize the separation of m-cresol and p-cresol isomers, but the analysis time of this method is 35 minutes, which is not conducive to batch production sample analysis

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