Schistosoma japonicum thioredoxin glutathione reductase gene and DNA (deoxyribonucleic acid) vaccine thereof

A reductase, anti-schistosomiasis technology, applied in the field of bioengineering, can solve problems such as reinfection that cannot be solved

Inactive Publication Date: 2013-06-05
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, drugs, such as praziquantel, are used to treat people or animals already infected with schistosomiasis, but this does not solve the problem of reinfection
There is no report about the thioredoxin glutathione reductase gene and its DNA vaccine of Schistosoma japonicum

Method used

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  • Schistosoma japonicum thioredoxin glutathione reductase gene and DNA (deoxyribonucleic acid) vaccine thereof
  • Schistosoma japonicum thioredoxin glutathione reductase gene and DNA (deoxyribonucleic acid) vaccine thereof
  • Schistosoma japonicum thioredoxin glutathione reductase gene and DNA (deoxyribonucleic acid) vaccine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Cloning of Schistosoma japonicum Thioredoxin Glutathione Reductase SjTGR Gene and Construction of SjTGR-pVAX1 Recombinant Plasmid

[0022] 1.1 Experimental animals Male New Zealand white rabbits, weighing 2.5kg to 3kg, were purchased from Shanghai Luojing Feida Experimental Animal Farm.

[0023] 1.2 Adult Schistosoma japonicum (strain from mainland China) The cercariae of Schistosoma japonicum were provided by the snail room of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. New Zealand white rabbits were infected with 2000 cercariae of Schistosoma japonicum by abdominal patch method, and were dissected 42 days after infection. Adult worms of Schistosoma japonicum were collected from the hepatic portal vein by thoracic aorta perfusion method, and the worms were fully washed with sterile PBS to remove the adhesion from the host. substance. The washed adult schistosomes were frozen in liquid nitrogen for later use.

[0024] 1....

Embodiment 2

[0056] Example 2 Evaluation of the immune protection effect of the recombinant plasmid SjTGR-pVAX1 in the mouse schistosomiasis model

[0057] 1. Materials

[0058] Male BALB / c mice aged 5-6 weeks were purchased from the Department of Experimental Animal Science, Fudan University. The cercariae of Schistosoma japonicum strains from mainland China were provided by the Snail Laboratory of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0059] Reagents and equipment related to the gene gun were purchased from bio-rad company, and the operation was also carried out according to its instructions. Other reagents are the same as above.

[0060] 2. DNA vaccine preparation and immunization method

[0061] (1) First determine that the amount of metal particle carrier (MLQ) is 0.5 mg / bullet, and the loading ratio (DLR) of DNA is 5 μg / mg gold powder.

[0062] (2) Coating DNA onto the metal particle carrier.

[0063] 1) Take the PVP (Polyvinylpyrrol...

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PUM

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Abstract

The invention discloses a schistosoma japonicum gene, which is a schistosoma japonicum thioredoxin glutathione reductase gene with an amino acid sequence shown as SEQ ID NO.1. The invention also discloses application of the gene in preparing vaccine in preventing or treating schistosomiasis. The DNA (deoxyribonucleic acid) vaccine of the schistosoma japonicum thioredoxin glutathione reductase gene has good immunity prevention effect to the schistosomiasis, and is suitable to be used as a new vaccine for resisting the schistosomiasis.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a Schistosoma japonicum thioredoxin glutathione reductase gene (SjTGR gene for short) and a DNA vaccine thereof. Background technique [0002] Schistosomiasis is a parasitic disease that can infect both humans and animals. At different stages of schistosomiasis development, cercariae, juveniles, adults and eggs can cause different damages and complex immunopathological responses to the host. The epidemic range is wide. It can cause serious harm to both humans and domestic animals. At present, drugs, such as praziquantel, are mainly used to treat people or animals already infected with schistosomiasis, but this cannot solve the problem of reinfection. In order to prevent and control schistosomiasis more effectively, a more feasible measure is to use a combination of vaccine and drug treatment. Therefore, the development of new anti-schistosomiasis vaccines is still one of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/63A61K48/00A61K39/00A61P33/12C12N5/10C12N1/21
CPCY02A50/30
Inventor 傅志强林矫矫曹燕韩艳辉赵彬石耀军张娟吴秀娟洪炀艾德宙朱珠
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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