Anti-tumor effective part of traditional Chinese medicine lespedeza as well as preparation method and application thereof

A technology of effective parts and twigs, applied to the effective antitumor parts of traditional Chinese medicine twigs and the fields of preparation and use thereof, can solve problems such as the research and application of anti-tumor effects of twigs that have not yet been seen

Inactive Publication Date: 2012-04-11
安徽省食品药品检验研究院 +1
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  • Abstract
  • Description
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  • Application Information

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At present, there is no report on the research and application of t

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  • Anti-tumor effective part of traditional Chinese medicine lespedeza as well as preparation method and application thereof
  • Anti-tumor effective part of traditional Chinese medicine lespedeza as well as preparation method and application thereof
  • Anti-tumor effective part of traditional Chinese medicine lespedeza as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0020] The preparation method of each effective part of Huzhizi:

[0021] The roots of the branches of Erudendron edulis were extracted 4 times with 50-80% ethanol solution in mass percent concentration, each time for 1.5 hours, and the extracts were combined to obtain F. After the ethanol is recovered, the aqueous solution is left to settle and precipitated. After the precipitation is complete, it is centrifuged to obtain the effective part A of the precipitate. The aqueous solution after centrifugation is extracted with ethyl acetate to obtain the effective part B; then extracted with n-butanol to obtain the effective part C; the remaining aqueous solution is concentrated and dried Get the effective part D.

Embodiment 2

[0023] Anti-blood tumor experiment:

[0024] Its representative experiment is that different effective fractions of Rhododendron chinensis inhibit the growth of T lymphoma EL-4 cells in mice. The method is aseptic subcutaneous inoculation of EL-4 cells in the right armpit of C57BL / 6 mice. After the EL-4 cells are stably passaged in the mice, the tumor tissue is subcutaneously removed from the axilla under aseptic conditions. A tumor cell suspension was made, and 0.2 mL of the above tumor cell suspension was inoculated subcutaneously in the right armpit of C57BL / 6 mice. The inoculated tumor-forming mice were randomly divided into groups, and the effective parts of the extracts of Prunus chinensis were given to groups A, B, C and D by intragastric administration, respectively, at the doses of 0.7, 0.18, 0.28, and 0.95 g / kg, and the administration was continued for 30 days. The tumor inhibition rate of the mice in the drug group. The results are shown in Table 1. Different effe...

Embodiment 3

[0029] Anti-hysteromyoma experiment: The experimental uterine fibroid model in rats was established by estrogen and progesterone loading method, and SD rats were selected and randomly divided into two groups: a uterine fibroid model group and a blank control group. The model group was given intramuscular injection of estradiol benzoate 0.06mL / rat, 3 times / week, for a total of 16 weeks, and progesterone 0.05mL / rat was added from the 10th week, 2 times / week, for a total of 7 weeks. After the modeling was completed, the effective fractions extracted from Prunus chinensis A, B, C, and D were administered intragastrically in groups, at 0.6, 0.14, 0.23, 0.91, and 2.0 g / kg, respectively, for a total of 30 days. Two hours after the last administration, the rats were sacrificed by cervical dislocation, and the uteri were removed, fixed in formalin, and subjected to HE staining for histomorphological observation. Scoring criteria for the degree of smooth muscle cell proliferation: (-): ...

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Abstract

The invention relates to an anti-tumor effective part of lespedeza extract and a preparation method thereof. The effective part is selected from one or the combination of a part A, an ethyl acetate part B, a normal butyl alcohol part C and a part D, wherein the part A is obtained by precipitating water solution of extracting solution extracted from lespedeza root under reflux of ethanol after ethanol recovery, the ethyl acetate part B is obtained by extracting the water solution with ethyl acetate, the normal butyl alcohol part C is obtained by extracting the water solution extracted by ethyl acetate with normal butyl alcohol, and the part D is finally obtained by concentrating and drying the treated water solution. Researches prove that the part can treat blood tumor, hysteromyoma, breast cancer and oophoroma. Therefore, the effective part can be used for preparing anti-tumor medicaments.

Description

1. Technical field [0001] The invention relates to an active part in a plant Chinese herbal medicine, an extraction method and application thereof, in particular to an anti-tumor effective part of the traditional Chinese medicine Echidda chinensis, a preparation method and an application thereof. 2. Background technology [0002] There are more than 60 species of plants of the genus Lespedeza Michx. in the leguminous family, and 26 species are produced in China, which are widely distributed in various provinces and autonomous regions of the country; the medicinal value of plants of the genus Lespedeza Michx. , diuretic swelling, promoting blood circulation and relieving pain, anti-inflammatory, anti-allergic, anti-early pregnancy. [0003] Tumor is one of the major diseases that endanger human health, and the prevention and treatment of malignant tumors has become an important topic of concern in the medical field. [0004] In recent years, although modern medicine has made...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P35/00A61K125/00
Inventor 阚红卫王吉东张毅梁燕汪春彦赵蓉王海青石静丽
Owner 安徽省食品药品检验研究院
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