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Culture medium for in vitro culture of bovine embryo

A technology of in vitro culture and culture medium, applied in the field of culture medium for in vitro culture of bovine embryos, can solve the problem of low growth rate, and achieve the effect of high development rate and increased total number

Inactive Publication Date: 2012-07-11
LANNUO BIOTECH WUXI
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AI Technical Summary

Problems solved by technology

[0002] The existing medium for culturing bovine embryos in vitro generally selects the CR1aa medium containing bovine serum albumin (BSA), but when the existing medium cultures bovine embryos in vitro, the total blastocyst development rate and high The total number of high-quality blastocysts (C1 blastocysts, IETS standard) is not high. For this reason, researchers have conducted a large number of experiments. For specific experimental data, see figure 1 , which confirmed that sodium citrate and BSA have a beneficial effect on the in vitro development of bovine IVF embryos, and also determined that different embryonic nutrient factors contained in BSA and serum, probably not sodium citrate, had a positive effect on the promotion of bovine morula and blastocysts. Embryo development, so it was found that this embryo nutrient factor is crucial for the composition of the medium for culturing bovine embryos in vitro

Method used

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  • Culture medium for in vitro culture of bovine embryo
  • Culture medium for in vitro culture of bovine embryo
  • Culture medium for in vitro culture of bovine embryo

Examples

Experimental program
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Effect test

Embodiment Construction

[0021] Experiment 1: Effect of medium containing divalent magnesium ions on IVF embryos co-cultured with somatic cells

[0022] Such as figure 2 As shown, the serum-free CR1aa medium added with BSA was divided into five groups A-E to culture bovine embryos in vitro, in which group A did not add divalent magnesium ions, and groups B, C, D, and E added 0.35 mM, 0.70 mM, 1.4 mM , 2.1 mM divalent magnesium ions, and the five groups A-E showed that the active agent was 6 mg / ml ICPbio BSA. In the same other environment, they were repeated four times to obtain the experimental data. figure 2 It shows that adding divalent magnesium ions from 0.35mM to 2.1mM in the medium can significantly improve the quality of embryo culture from the mulberry stage, and the number and quality of embryos cultivated under the relative 0.35mM concentration under the concentration of 1.4mM are higher than those under the concentration of 1.4mM. Significantly increased.

[0023] Experiment 2: Effe...

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Abstract

The invention provides a culture medium for in vitro culture of a bovine embryo. According to the culture medium, a new embryo nutrient factor is added, such that the total blastula developmental rate of the in vitro bovine embryo cultured in the culture medium of the present invention is high, and the number of the high quality blastulas is increased. The culture medium of the present invention is characterized in that bivalent magnesium ions are added to the culture medium.

Description

technical field [0001] The invention relates to the technical field of culturing bovine embryos in vitro, in particular to a culture medium for cultivating bovine embryos in vitro. Background technique [0002] The existing medium for culturing bovine embryos in vitro generally selects the CR1aa medium containing bovine serum albumin (BSA), but when the existing medium cultures bovine embryos in vitro, the total blastocyst development rate and high The total number of high-quality blastocysts (C1 blastocysts, IETS standard) is not high. For this reason, researchers have conducted a large number of experiments. For specific experimental data, see figure 1 , which confirmed that sodium citrate and BSA have a beneficial effect on the in vitro development of bovine IVF embryos, and also determined that different embryonic nutrient factors contained in BSA and serum, probably not sodium citrate, had a positive effect on the promotion of bovine morula and blastocysts. Embryo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 杜福良徐捷薛非杨澜
Owner LANNUO BIOTECH WUXI
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