Determination method for ammonia (ammonia ions) and ammonia (ammonia ion) diagnostic/assay kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0129] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0130] 1) Preparation of standard samples
[0131] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0132] 2) Pretreatment of samples to be tested
[0133] Plasma samples are used as samples to be tested without pretreatment;
[0134] 3) Blank sample
[0135] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0136] B. Preparation of reagent solution:
[0137] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0138] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0139] Glycerin 1mol / L
[0140] NADPH0.25mmol / L
[0141] Ammonia kinase 16000U / L
[0142] Dethiobiotin synthase 18000U / L
[0143] Malate dehydrogenase 12000U / L
[0144] Adenosine triphosp...
Embodiment 2
[0160] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0161] 1) Preparation of standard samples
[0162] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0163] 2) Preparation of samples to be tested
[0164] Plasma samples are used as samples to be tested without pretreatment;
[0165] 3) Blank sample
[0166] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0167] B. Preparation of reagent solution:
[0168] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0169] Reagent 1 with the following composition:
[0170] Phosphate buffer 100mmol / L
[0171] NADH0.25mmol / L
[0172] Adenosine triphosphate 5mmol / L
[0173] Desthiobiotin 5mmol / L
[0174] Monovalent phosphate 5mmol / L
[0175] Pyruvate 5mmol / L;
[0176] Re...
Embodiment 3
[0198] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0199] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0200] B. Preparation of reagent solution:
[0201] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0202] Reagent 1 with the following composition:
[0203] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0204] NADH0.25mmol / L
[0205] Adenosine triphosphate 5mmol / L
[0206] Desthiobiotin 5mmol / L
[0207] Monovalent phosphate 5mmol / L
[0208] Pyruvate 5mmol / L;
[0209] Reagent 2 with the following composition:
[0210] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0211] Sodium chloride 2mol / L
[0212] Dethiobiotin synthase 28000U / L
[0213] Malate dehydrogenase 50000U / L;
[0214] Reagent 3 with the following composition:
[0215] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L ...
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