Multifunctional container for freezing cells and resurrecting frozen cells

A multi-purpose, cell-based technology, applied in the field of medical devices, can solve the problems of increased frequency of exposure to air, inconsistent operation process, low recovery rate, etc., and achieve the effect of cell quality assurance, perfect processing process, and guarantee of clinical effect

Active Publication Date: 2013-09-25
BEIJING RUIJIANTIANXING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The widely used clinical material for storing cells in liquid nitrogen is the cell storage bag of Miltenyi Biotec, which has been approved by the U.S. FDA and approved by the Chinese Drug Administration; 1. Each stage of resuscitative centrifugation needs to use different containers, resulting in increased exposure to air and increased chances of cell product contamination
In addition, when used by clinicians, cells need to be aspirated into different containers. The aspiration process may be polluted and some cells may be destroyed.
[0007] The inventors of the present invention aimed at the technical problems existing in the prior art, such as low recovery rate, non-uniform operation process and the above-mentioned frozen cells being easily polluted and damaged, through a careful study of the whole process of cell recovery after freezing , design a new type of cell freezing and recovery container

Method used

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  • Multifunctional container for freezing cells and resurrecting frozen cells
  • Multifunctional container for freezing cells and resurrecting frozen cells

Examples

Experimental program
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Effect test

Embodiment 1

[0029] like figure 1 As shown, this embodiment relates to a multi-purpose cell freezing and resuscitating container after cryopreservation, including a cryopreservation bag, which has an oval bottom and a rectangular upper part in an empty state, and the thickness of the cryopreservation bag is 250 μm. The cryopreservation bag is composed of Ethylene-vinyl acetate (EVA) is made; The bag mouth of described cryopreservation bag is also connected with lavage and output channel, cell input pipeline and washing solution inlet pipeline. The end of the cell input pipeline and the cleaning solution inlet pipeline are connected with Luer bolts; the oval bottom of the cryopreservation bag has a thickened layer, the thickness of which is 100 μm, and the thickened layer is also made of EVA; the cryopreservation bag also has a label bag and infusion hanging hole; the mouth of the cryopreservation bag is also connected with a lavage and output channel, which is used to connect with the lava...

Embodiment 2

[0032] This embodiment relates to a multi-purpose cell freezing and resuscitation container after cryopreservation, including a cryopreservation bag with an oval bottom and a rectangular upper part in an empty state, the thickness of the cryopreservation bag is 300 μm, and the cryopreservation bag is made of ethylene-acetic acid It is made of vinyl ester (EVA); the mouth of the cryopreservation bag is also connected with lavage and output channels, cell input pipelines and cleaning solution inlet pipelines. The end of the cell input pipeline and the cleaning solution inlet pipeline are connected with Luer bolts; the thickness of the thickened layer is 150 μm, and the thickened layer is made of ethylene-methacrylate; the cryopreservation bag also has a label bag and an infusion hanging hole; The mouth of the bag is also connected with a lavage and output channel, which is used to connect with the lavage solution device, and the lavage and output channel is closed with a protecti...

Embodiment 3

[0035] This embodiment relates to a multi-purpose cell freezing and resuscitation container after cryopreservation, including a cryopreservation bag with an oval bottom and a rectangular upper part in an empty state, the thickness of the cryopreservation bag is 300 μm, and the cryopreservation bag is made of ethylene-acetic acid It is made of vinyl ester (EVA); the mouth of the cryopreservation bag is also connected with lavage and output channels, cell input pipelines and cleaning solution inlet pipelines. The end of the cell input pipeline and the cleaning solution inlet pipeline are connected with Luer bolts; the thickness of the thickened layer is 150 μm, and the thickened layer is made of ethylene-methacrylate; the cryopreservation bag also has a label bag and an infusion hanging hole; The mouth of the bag is also connected with a lavage and output channel, which is used to connect with the lavage solution device, and the lavage and output channel is closed with a protecti...

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Abstract

The invention relates to a multifunctional container for freezing cells and resurrecting frozen cells. The multifunctional container comprises a freezing bag, wherein the freezing bag is provided with an elliptical bottom and a rectangular upper part in an idle state. The multifunctional container is characterized in that a thickening layer is arranged at the elliptical bottom of the freezing bag; and a lavage and output channel, a cell input pipeline and a cleaning liquid entrance pipeline are also connected to the bag mouth of the freezing bag. The multifunctional container for freezing the cells and resurrecting the frozen cells has the advantages that the effect of multifunction by one bag is achieved; the preparation process of the cells and clinical use are facilitated; more importantly, the possibility that the cells are in contact with air and are polluted is greatly reduced; and the quality control over the cells and the purity of products are facilitated.

Description

technical field [0001] The invention relates to a medical device, in particular, the invention relates to a container used for freezing, resuscitating and infusion of cells. Background technique [0002] With the development of biological technology, cell therapy is developing rapidly and being accepted by people all over the world. Fundamental to cell therapy is the acquisition and storage of living cells. In addition, the in vitro culture of cells has also been widely used in various biological research fields. In in vitro cell culture work, in order to preserve cells and make them biologically active for a long time, cell cryopreservation and recovery have become crucial links. The preparation and storage of cells, especially the low-temperature storage of stem cells, the recovery conditions and the prevention of contamination during the operation process are important issues in the clinical application of cell therapy. Many countries and regions have established vario...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/00A01N1/02
Inventor 胡敏
Owner BEIJING RUIJIANTIANXING BIOTECH
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