Sample containing tuberculosis serum characterized protein and preparation method thereof

A technology for serum protein, active tuberculosis, applied in the biological field

Active Publication Date: 2012-09-12
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Sample containing tuberculosis serum characterized protein and preparation method thereof
  • Sample containing tuberculosis serum characterized protein and preparation method thereof
  • Sample containing tuberculosis serum characterized protein and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0026] Example 1 The difference between normal and pulmonary tuberculosis patients and the detection of mass spectrometry

[0027] (1) Experimental method

[0028] 180 cases of pulmonary tuberculosis patients, the average age was 43.8 ± 12.2 (18-65 years old). Tuberculosis patients had no patients with liver, kidney, metabolic autoimmune diseases, endocrine, blood, nervous system diseases, malignant tumors, or long-term use of immunosuppressants. 120 controls with other pulmonary diseases (including 40 cases of lung cancer, 40 cases of pneumonia, and 40 cases of COPD), were excluded from clinical diagnosis of tuberculosis. The 91 healthy volunteers were normal people with normal physical examination. The average age of the non-tuberculosis group was 46.3±14.1 (19-65 years old). Subjects collected 1 mL of veins on an empty stomach, immediately after collection, put them in a refrigerator at 4°C for 2 hours, centrifuged at 4°C at 4,000r / min for 10 minutes to separate serum, an...

Embodiment 2

[0043] Embodiment 2 clinical trial and double-blind test

[0044] Since the combination of multiple characteristic proteins can completely separate pulmonary tuberculosis from normal people, so any two or more of the above 35 characteristic proteins are selected, and according to the mass-to-charge ratio m. / z value of each protein peak and the critical peak value M of the protein to establish a serum characteristic protein profile model for the identification of tuberculosis patients and non-tuberculosis patients ( Figure 5 ), the m / z value of the specific protein mass-to-charge ratio and the critical peak value M of the protein are respectively m / z=5325.7, M=5.77; m / z=8608.8, M=2.80; m / z=4824.4 ,M 1 =6.70, M 2 =2.43; m / z=2554.6, drawn by M=3.37; molecular weight (m / z) error<0.01%, CV of critical peak mean value M<5-10%. Using the mass spectrometry model, as long as the mass-to-charge ratio of the corresponding protein in the subject's serum and the critical peak value M o...

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Abstract

The invention relates to a sample containing a tuberculosis serum characterized protein and a preparation method thereof. The sample comprises the serum of an untreated patient suffering from active tuberculosis, and the serum protein of a patient suffering from lung cancer, a patient suffering from pneumonia, a patient suffering from chronic obstructive pulmonary disease or a healthy person who is ensured to be normal by physical examination, wherein the serum protein comprises three up-regulated proteins and one down-regulated protein; the proteins exit independently or any or all of the proteins are mixed together; and the sample is suitable to be detected by using mass spectrum. The sample provides a basis for further discovering new active tuberculosis biomarkers. By using the sample, the detection of the active tuberculosis is superior to any single detection method adopted currently; a non-invasive technology for early discovery and early treatment of the active tuberculosis is provided; and therefore, a new method for reducing the infectious rate of the active tuberculosis and improving the recovery rate of the tuberculosis is provided.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a sample containing tuberculosis serum characteristic protein and its preparation method and application. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis mainly caused by respiratory system infection, and it is a serious infectious disease affecting human health around the world. The high morbidity and mortality of tuberculosis, as well as airborne transmission, imposes a considerable burden on the patient, the things the patient is exposed to, and society, so early diagnosis plays a vital role in the control of tuberculosis. However, the current diagnostic techniques for pulmonary tuberculosis are not ideal. Mycobacterium tuberculosis culture has high sensitivity and specificity, but it takes a long time to cultivate, which increases the risk of exacerbation and transmission of tuberculosis; rapid methods such as ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N27/62
Inventor 李继承刘姬燕
Owner ZHEJIANG UNIV
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