Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
A tolerance and fixation rate technology, applied in the field of microalgae with high CO2 tolerance and fixation rate and its breeding field, can solve problems such as unsuitability, achieve good adaptability, good social and environmental benefits, and reduce complexity Effect
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[0038] The first stage: Screening, domestication and isolation and purification of starting strains
[0039] The test device is attached figure 1 shown. The test samples are as follows: a paddy field soil sample (moisture content ≥ 90%), a hot spring pond water sample, a hot spring natural river water sample, a hot spring sewage pond water sample, a water pool near a power plant and a power plant section water sample .
[0040] Sample pre-incubation phase:
[0041] BBM medium, the composition of which is shown in Table 2, has a pH of about 6.6. The prepared mother solution was sterilized separately and stored at 4°C. When in use, add it to distilled water in sequence according to the mother liquor, mix evenly and set the volume to the mark.
[0042] The composition of table 2BBM medium
[0043]
[0044] Take a small amount of water samples and soil samples from each sampling source, dissolve them in water, and take the supernatant. In a 300ml Erlenmeyer flask, the sa...
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