Method for conservation in vitro of cassava germplasm resources with stability and high efficiency

A technology of in vitro preservation and cassava, applied in the field of agricultural biology, can solve the problems of prolonging the generation cycle, high energy consumption, high equipment cost, etc., and achieve the effect of energy saving and simple process flow

Active Publication Date: 2012-11-21
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

As we all know, low-temperature or ultra-low temperature storage equipment costs a lot and consumes a lot of energy. How to make the preserved germplasm grow slowly under normal temperature culture conditions (25±2℃, 3000xl light intensity, 16h / 8h photoperiod) to extend the subculture cycle , the purpose of reducing the cost of cultivation is a major task of the current in vitro preservation technology of germplasm resources

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  • Method for conservation in vitro of cassava germplasm resources with stability and high efficiency

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Embodiment Construction

[0015] In order to describe in detail the technical content and structural features of the stable and efficient in vitro preservation method for cassava germplasm resources of the present invention, the following will be further described in conjunction with the embodiments.

[0016] A stable and efficient method for in vitro preservation of cassava germplasm resources, including explant disinfection treatment of in vitro preservation materials, acquisition of sterile test tube seedlings, and subculture of test tube seedlings. The specific steps are as follows:

[0017] 1. Disinfection of explants

[0018] In the field or laboratory potted seedlings, cut cassava shoots with buds, remove the leaves and part of the petiole, soak in washing powder for 20 minutes, then rinse with tap water for 20 minutes, soak in 75% alcohol for 8 seconds on the ultra-clean workbench, and then Soak in 0.5% mercury liter (HgCl2) solution for 10-15 minutes, and rinse with sterile water 4-5 times.

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Abstract

The invention discloses a method for conservation in vitro of cassava germplasm resources with stability and high efficiency, characterized by sub-culturing an aseptic test-tube plantlet in a growth inhibition medium containing kanamycin having a certain concentration, wherein the formula comprises MS, 0.2mg/L NAA, 80mg/L Kana, 30g/L cane sugar and 9g/L agar, and the pH value is 5.8; under the condition of no pollution, the test-tube plantlet can grow normally and is conserved for more than 22-24 months which is 10-12 times the conservation time of the medium with no addition of growth inhibition substances, the test-tube plantlet growing in the growth inhibition medium has a developed root system, significantly shortened intemode, significantly increased stalk pitch number, obviously thickened stalk, and deeper green leaves, and especially the greenhouse transplanting survival rate reaches more than 90% which is 5-8 times that of the control groups.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a stable and efficient method for in vitro preservation of cassava germplasm resources. Background technique [0002] Cassava is Euphorbiaceae (Euphorbiaceae), cassava (Manihot) plants, the genus has 98 species, of which only one cultivated species of cassava (Manihot esculenta Crantz), aliases cassava, cassava, Yuca, Tapioca and Manioc, English named Cassava. It has been cultivated in the Americas for 5,000 years. It is one of the three largest potato crops in the world. The planting area is about 18 million hectares, second only to potatoes and larger than sweet potatoes. It is distributed between 30° north and south latitudes and below 2,000 meters above sea level. So far, cassava has been widely planted in 95 countries and regions in Africa (49%), Asia (28%) and America (23%), becoming an important tropical crop to solve hunger and poverty, and providing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 欧文军李开绵李庚虎陈松笔叶剑秋
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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