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Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology

A Melaleuca tower and supercritical technology, applied in the field of huperzine A, can solve the problems of small production scale, failure to meet market demand, troublesome operation, etc.

Inactive Publication Date: 2014-11-05
HENAN HAIYUESEN PHARMA CELL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Above-mentioned various methods or the product purity that obtains is lower, or yield is lower, or operation is troublesome, and the production cost that has is higher, and production scale is small, can't satisfy market demand

Method used

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  • Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology
  • Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology
  • Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: get melaleuca pagoda coarse powder 10kg, add 1% hydrochloric acid solution 20kg, soak overnight, ultrasonic 120S, be entrainer with ethanol, supercritical extraction, obtain relative density and be 1.26 (60 ℃ of thermal measurements) 2.41kg.

Embodiment 2

[0038] Embodiment 2: get melaleuca tower coarse powder 10kg, add 1% hydrochloric acid solution 30kg, soak overnight, ultrasonic 240S, be entrainer with ethanol, supercritical extraction, obtain relative density and be 1.25 (60 ℃ of thermal measurements) 2.46kg.

Embodiment 3

[0039] Embodiment 3: get melaleuca coarse powder 10kg, add 1% tartaric acid solution 20kg, soak overnight, ultrasonic 120S, be entrainer with ethanol, supercritical extraction, obtain relative density and be 1.27 (60 ℃ of thermal measurements) 2.44kg.

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Abstract

The invention discloses a method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by a supercritical extraction-crystallization technology. The method is characterized in that the method comprises material preparation, microwave radiation, extraction, crystallization, recrystallization, product detection and packaging; through utilization of a microwave radiation wall-breaking technology, a use amount of an acid used by the traditional extraction method, acid discharge and environmental pollution are reduced; through utilization of a supercritical extraction-crystallization technology, a yield is greatly improved; a small amount of cheap ethanol is used as a solvent so that a production cost is greatly reduced; and compared with a production cost of the traditional technology, a production cost is reduced by 40%. Through a supercritical grading crystallization process on extract, structural identification and purity detection prove that five high-purity alkaloids are separated out. Raw materials having huperzine A content more than 0.0025% have industrial production values so that maximum resource utilization is realized. Waste CO2 discharged by supercritical extraction is subjected to condensation compression, then enters into a primary extraction kettle with carrying ethanol as an entrainer and is extracted again so that a CO2 use amount is reduced.

Description

technical field [0001] The invention relates to a method for extracting and separating huperzine A in melaleuca tower and its hairy roots by using supercritical extraction-crystallization technology. technical background [0002] Melaleuca pagoda is the whole herb of the genus Hupermus serrata in the family Huperdaceae. It is used in folks to dissipate fatigue and blood, reduce swelling and relieve pain, dehumidify, clear away heat and detoxify. The alkaloids huperzine A and huperzine B contained in it have a strong inhibitory activity on cholinesterase, and clinical experiments have confirmed that huperzine A has a significant effect on the treatment of Alzheimer's disease. Huperzine A extracted from Melaleuca pagoda is a world-class new drug created in China. Belonging to reversible cholinesterase inhibitors, it is currently one of the safest and most effective drugs for the treatment of benign memory impairment and Alzheimer's disease. [0003] There are existing litera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/22C07D471/08C07D455/03C07D487/08
CPCY02P20/54
Inventor 张宗申于振艳刘同祥李志成郭奇根
Owner HENAN HAIYUESEN PHARMA CELL ENG TECH
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