Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus pine polysaccharide and its application

A technology of pine milk mushroom polysaccharide and pine milk mushroom, which is applied in the direction of drug combination, antitumor drug, etc., can solve the problems that the structure and activity of pine milk mushroom polysaccharide have not been reported.

Inactive Publication Date: 2016-04-27
CHINA WEST NORMAL UNIVERSITY
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there has not been any report on the structure and activity research and application of Lactobacillus pine polysaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus pine polysaccharide and its application
  • Lactobacillus pine polysaccharide and its application
  • Lactobacillus pine polysaccharide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The separation and extraction of embodiment 1 milk mushroom polysaccharide

[0018] Commonly used methods for extracting polysaccharides from large edible and medicinal fungi mainly include solvent extraction, ultrasonic extraction, microwave extraction, supercritical extraction, and enzymatic extraction. After impurity removal, crushing, pretreatment, hot water extraction and alcohol precipitation treatment, the crude extract of lactobacillus pine polysaccharide is obtained. We use DEAE-cellulosecolumn and SephadexG-100column for separation and purification. The process of extraction, separation and purification is as follows: raw material → crushing → degreasing → solvent extraction → extract → centrifugation → concentration → dialysis → alcohol precipitation → protein removal → crude polysaccharide → column chromatography → distribution collection → concentration and drying → purity identification.

[0019] 1.1 Materials

[0020] 1.1.1 Strains

[0021] Fresh fruit...

Embodiment 2

[0073] Example 2 Study on Antitumor Activity of Lactarius pine polysaccharides

[0074] To study the immunomodulatory activity of lactobacillus polysaccharides, to provide a theoretical basis for the application and promotion of lactobacillus polysaccharides. In vivo, we tested its inhibitory effect on mouse S180 solid tumor by intraperitoneal injection of lactobacillus polysaccharides to detect its anti-tumor activity.

[0075] 2.1 Materials

[0076] 2.1.2 Reagents

[0077] mannan peptide

[0078] 2.1.3 Instruments

[0079] Biological anatomy mirror: Hunan Changsha Chuang Hi-Tech Co., Ltd.; DZF-200 vacuum drying oven: Shanghai Pudong Dongrongfeng Scientific Instrument Co., Ltd. Shanghai Pudong Yuexin Scientific Instrument Factory; SH21-1 constant temperature magnetic stirrer: Shanghai Meiyingpu Instrument Instrument Manufacturing Co., Ltd.; J-25I high-speed refrigerated centrifuge: BeckmanCoulter, the United States; PA92-F112I electronic analytical balance: SartoriusLimit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a structure of lactarius deliciosus polysaccharide and an application thereof; the lactarius deliciosus polysaccharide is heteropolysaccharide composed of two monosaccharides which are alpha-L-mannose and alpha-D-xylose with a ratio of 3:1; the lactarius deliciosus polysaccharide has an average molecular weight of 1.1*10<4> D, has a (1-6) alpha-L-mannose skeleton; a -3)-alpha-D-xylose side chain is connected with 2-O; a certain amount of lactarius deliciosus polysaccharide can significantly inhibit the growth of transplanted tumor S180. Groups with intraperitoneal injection of 20 mg / Kg.d, 40 mg / Kg.d, and 80 mg / Kg.d (L1, L2, L3 group) respectively have a tumor inhibition rate of 44.358%, 52.326%, and 68.422%; a positive control group with a concentration of 20 mg / Kg has a mannatide (M) tumor inhibition rate of 57.727%; no obvious damage, and no toxic or side effect are caused to mouse visceral organs; and the immunity is significantly improved.

Description

technical field [0001] The invention relates to the technical field of fungal polysaccharide extraction and application, in particular to lactobacillus pine polysaccharide and application thereof. Background technique [0002] Fungal polysaccharides generally refer to a class of metabolites produced by the fruiting bodies and mycelium of various fungi. They can be divided into pure polysaccharides and heteropolysaccharides. Pure polysaccharides are generally composed of more than 10 monosaccharides linked by glycosidic bonds. , can be divided into linear structure and branched structure. In addition to sugar chains, heteropolysaccharides can also contain peptide chains or lipid components. Fungal polysaccharides, also known as polysaccharides, are a class of biomacromolecules ubiquitous in biological organisms. They not only participate in the formation of tissue cytoskeleton, but also are important components of various endogenous bioactive molecules. Before the 1960s, su...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 丁祥侯怡铃侯万儒
Owner CHINA WEST NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com