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Application of metabolins of streptomyces corchorusii NF0919 bacterial strain in prevention and control cucumber diseases

A technology of Streptomyces jute, metabolites, applied in application, chemicals for biological control, fungicides, etc., can solve the problems of not mentioned and disclosed

Inactive Publication Date: 2013-01-16
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Abstract
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Problems solved by technology

[0004] The antagonistic bacterium Streptomyces jute NF0919 strain that can simultaneously cure cucumber fungus and oomycete diseases provided by the present invention is not mentioned and disclosed in the prior art

Method used

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  • Application of metabolins of streptomyces corchorusii NF0919 bacterial strain in prevention and control cucumber diseases

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Embodiment

[0019] For the strain characteristics of the Streptomyces juteus NF0919 strain provided in the present invention, see patent ZL201010236886.1. The extraction method of the product in the fermentation liquid of Streptomyces juteus NF0919 strain of the present invention comprises the following steps:

[0020] (A) Activation of strains and cultivation of primary seed broth

[0021] The slant of the preserved NF0919 strain was activated on Gao's No. 1 medium, cultivated at 30 °C for 10 d, and picked 1 cm 2 The size of the bacterial mass was inoculated into 50 mL of primary seed culture solution (50 mL of culture solution in a 250 mL triangular flask), placed on a 170 rpm shaker at 30 °C for 44 to 48 hours of shaking culture, wherein the 50 mL of primary seed cultured The composition of the liquid is: soluble starch 3%, glucose 2%, yeast powder 1%, peptone 1%, NaCl 0.5%, K 2 HPO 4 0.2%, soluble starch, glucose, yeast powder, peptone, NaCl and K 2 HPO 4 The unit ratio of th...

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Abstract

The invention relates to a method for extracting active metabolins in fermentation broth of streptomyces corchorusii NF0919 bacterial strain (whose preservation number is CGMCC (China General Microbiology Culture Center) No. 3968 and strain patent number is ZL201010236886.1). The metabolins are capable of effectively preventing and controlling Erysiphecichoracearum, Pseudoperonosporacubensis, Botrytiscinerea and Fusariumoxysporum.

Description

field of invention [0001] The present invention relates to a kind of microorganism and application, more specifically relate to a kind of Streptomyces jute ( Streptomyces corchorusii ) NF0919 strain metabolites and their application in the prevention and control of cucumber diseases belong to the technical field of biological pesticides. Background technique [0002] Cucumber powdery mildew ( Erysiphe cichoracearum ), cucumber downy mildew ( Pseudoperonospora cubensis ), Cucumber Botrytis ( Botrytis cinerea ) and cucumber wilt ( Fusarium oxysporum ) are the main fungal and oomycete diseases on cucumber. At present, the prevention and control of cucumber diseases is mainly through chemical control. The commonly used chemical agents are: triadimefon, sacralin smoke agent, oxaxyl, azoxystrobin, kresoxim-methyl, difenoconazole, enoxime esters, boscalid and pyraclostrobin, etc. For a long time, due to the frequent and multi-dose application of chemical agents, cucumber ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00
Inventor 杨敬辉陈宏州张文文庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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