9 results about "Erysiphe sp." patented technology

This invention relates to a powdery mildew resistance gene PmAeu1 derived from *Aegilops spp.*, its molecular markers, and applications, belonging to the field of genetic engineering. This invention utilizes the powdery mildew-resistant *Aegilops spp.* strain AE 1617 and the powdery mildew-susceptible strain AE 538 to construct mapping populations. Through BSR-Seq, fine genetic mapping, third-generation genome sequencing, and transgenic verification, a new powdery mildew resistance gene, PmAeu1, was cloned. This invention has demonstrated through transgenic wheat research that the PmAeu1 gene can significantly improve wheat resistance to powdery mildew. The *Aegilops spp.* powdery mildew resistance gene PmAeu1 can be applied to genetically improve wheat varieties to enhance wheat's powdery mildew resistance. This invention also develops a molecular marker, XAeuNLR1-2, for the PmAeu1 gene, which can be used to specifically detect the gene PmAeu1 as described in the claims and can be applied to marker-assisted breeding of the PmAeu1 gene.

The disclosure discloses a primer for amplifying a molecular marker of powdery mildew resistance gene PmDR776 of Triticum dicoccon Schrank, where the molecular marker primer includes a forward primer HENU654-F and a reverse primer HENU654-R; the nucleotide sequence of the forward primer HENU654-F is shown in SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU654-R is shown in SEQ ID NO:2. The molecular marker primer provided by the disclosure is applied to detection and identification of gene PmDR776 and auxiliary identification of a wheat powdery mildew resistance trait, and molecular marker-assisted selection breeding.

The application discloses application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew, and belongs to the field of genetic engineering, and particularly relates to application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew. The protein of the application is any one of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No: 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1), the protein having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The Pm61 protein can positively regulate the resistance of wheat to powdery mildew, and can be used for wheat breeding with resistance to powdery mildew.

Provided herein are powdery mildew-resistant grapevine plants and methods for providing powdery mildew resistance to susceptible grapevine plants. Specifically, provided herein are grapevine plants including in their genome an impaired Erysiphe necator resistance-conferring gene, wherein the corresponding not impaired Erysiphe necator resistance conferring resistance-conferring gene designated VvMLO13 encodes a protein including the amino acid sequence of SEQ ID No. 1, or proteins having 95% sequence identity therewith. The impairment results in an absence of a protein comprising the amino acid sequence of SEQ ID No. 1, or proteins having 95% sequence identity therewith, in the grapevine plant and wherein the grapevine plant is resistant to powdery mildew.

The application discloses a method for observing infection of wheat leaf by Erysiphe graminis. The back of the wheat leaf is fixed on an acrylic plate, and the spores of Erysiphe graminis are inoculated uniformly; the epidermis of the back of the leaf is torn and immersed in a phosphate buffer solution; the buffer solution is absorbed, a cell component eluent is added for elution, and then the phosphate buffer solution is replaced; the phosphate buffer solution is absorbed, and a coomassie brilliant blue solution is added for staining, so as to obtain a plant sample for observation. The method can greatly save the experimental time, the reagents used do not contain toxic reagents, the structure, edge and infection point of each period of pathogen infection are clearer, and the background color is uniform and clear. The method can be used for rapid observation of the infection of cereal crops by powdery mildew, and is also suitable for the observation of the infection of other plant pathogenic fungi.

The invention relates to a preparation method and application of dextran heptasaccharide, and belongs to the technical field of natural product synthesis. Compared with the reported preparation method of the dextran, the preparation method provided by the invention is a completely different preparation route, compared with the previous synthesis steps, the reaction of each step is optimized at present, the synthesis yield of each step of the glucose tetrasaccharide donor, the glucose trisaccharide receptor and the dextran is more than 90%, and the synthesis yield of each step of the dextran is more than 90%. Compared with the prior art, the synthesis yield is about 70-80% and is greatly improved, and remarkable technical progress is achieved. The dextran provided by the invention is effective in preventing and treating strawberry anthracnose and tomato bacterial wilt, has better effects on preventing and treating strawberry anthracnose and tomato bacterial wilt than the commercially available medicament amino-oligosaccharin, and has remarkable technical progress. The dextran provided by the invention is ineffective to melon powdery mildew, so that the dextran provided by the invention is not effective to all plant diseases, and through a large number of experiments, the dextran provided by the invention accidentally achieves a very remarkable effect on strawberry anthracnose and tomato bacterial wilt.

The disclosure discloses a primer for amplifying a molecular marker of powdery mildew resistance gene PmDR754 of Triticum dicoccon Schrank, where the molecular marker primer includes a forward primer HENU765-F and a reverse primer HENU765-R; the nucleotide sequence of the forward primer HENU765-F is shown in SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU765-R is shown in SEQ ID NO:2. The molecular marker primer provided by the disclosure is applied to detection and identification of gene PmDR754 and auxiliary identification of a wheat powdery mildew resistance trait, and molecular marker-assisted selection breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a CpWRKY71-related biological product in regulating and controlling powdery mildew resistance of summer squashes. The CpWRKY71 is any one of the following components: 1) a nucleotide sequence as shown in SEQ ID NO.29; 2) a sequence obtained by truncating SEQ ID NO.29, and the truncated sequence is shown as SEQ ID NO.31. According to the invention, the regulation and control effect of CpWRKY71 in summer squash on powdery mildew resistance is clear for the first time, the gene can specifically act on a signal channel of summer squash for dealing with powdery mildew stress, and the plant resistance is directionally enhanced by regulating and controlling the expression of downstream disease-resistant related genes.

This invention discloses a molecular marker, YTUL62, tightly linked to the wheat powdery mildew resistance gene PmL555. The upstream primer nucleotide sequence is shown in SEQ ID NO:1, and the downstream primer nucleotide sequence is shown in SEQ ID NO:2. Using the marker primers of YTUL62, PCR amplification of the wheat genomic DNA to be tested yielded amplification products with molecular weights of 226 bp and 260 bp, which are the molecular markers tightly linked to the wheat powdery mildew resistance gene PmL555. The molecular marker provided by this invention can more accurately and efficiently detect the genetic mapping population of PmL555, which is beneficial for map-based cloning and fine localization of PmL555. Furthermore, using this marker for marker-assisted selection of PmL555 can shorten the breeding cycle and improve breeding efficiency.