30 results about "Erysiphe sp." patented technology

The invention discloses a wheat anti-powdery mildew gene Pm21 linked codorminant PCR marker and its use, wherein disease-resistant gene analogues Ta-LRR2 sequence design primers NAU/XiBao 16F and NAU/XiBao 16R are obtained through suppression subtractive hybridization between Yangmai No.5 and translocation lines, a series of material tests have shown that, the label NAU/XiBao16 is interlocked with translocation lines 6VS/6AL powdery mildew resistant gene Pm21, and one PCR experiment can amplify two specific belts from common wheat 6AS and Haynaldia Vollosa 6VS chromosomes simultaneously, the label NAU/XiBao16 can be used for the molecular label auxiliary selection in wheat powdery mildew disease resistant breeding, and for the purity identification of common wheat seeds.

The invention discloses a method for quickly identifying a manihot esculenta mildew-resistance locus (MLO) gene, relates to knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The method mainly comprises the following steps of: 1) downloading a manihot esculenta whole genome sequence, and collecting the MLO gene; 2) identifying the MLO gene; 3) identifying the MLO gene according to the MLO gene phylogenetic relationship; and 4) comparing the MLO genes. By the method, the discovery cycle of the manihot esculenta MLO gene is shortened effectively, and the MLO gene can be quickly identified; corresponding coseparation functional markers (single nucleotide polymorphism (SNP) and specific combining ability (SCA)) can be developed by the identified candidate MLO gene, and the method can be quickly used for molecular marker auxiliary selection of the MLO gene, and is high in accuracy; by combining other disease-resistant gene molecular markers, multiresistance breeding materials can be prepared, the breeding cycle is shortened, and the breeding efficiency is improved; and the foundation is laid for elaborating a manihot esculenta MLO gene molecular mechanism.

The invention relates to a method for extracting active metabolins in fermentation broth of streptomyces corchorusii NF0919 bacterial strain (whose preservation number is CGMCC (China General Microbiology Culture Center) No. 3968 and strain patent number is ZL201010236886.1). The metabolins are capable of effectively preventing and controlling Erysiphecichoracearum, Pseudoperonosporacubensis, Botrytiscinerea and Fusariumoxysporum.

The invention discloses a wheat powdery mildew resistance gene and an application thereof. A wheat TaJAZ1 gene from an A genome is identified from a common wheat cultivar KN199 by the inventors, overexpression of TaJAZ1 missing a jas domain is proved to be capable of leading to wheat resistance enhancement through the stable transgenic technology, and positive regulation of wheat resistance by TaJAZ1 is indicated. The invention proves for the first time that TaJAZ1 is a key regulatory gene for wheat resistance.

The invention discloses ribosome RNA gene of erysiphe alphitoides and an application thereof. The nucleotide sequence of the ribosome RNA gene of the erysiphe alphitoides is shown in SEQ ID NO.1. Thesequence can be utilized for detecting the erysiphe alphitoides and also can be applied to study on sorting of fungal species. In addition, the invention further provides a group of primers with strong specificity for detecting the erysiphe alphitoides; based on the primers, a detection method and a detection kit for detecting the erysiphe alphitoides by the specificity are established and have good application prospect in detection of the erysiphe alphitoides.

The invention belongs to the field of genetic engineering, and discloses a gene TaHBP1 with a heme-binding domain, and a recombinant interference vector and an application thereof. The gene TaHBP1 with the heme-binding domain has the cDNA sequence of SEQ ID NO.1 and a coding amino acid sequence of SEQ ID NO.2. The gene comes from ordinary triticum asetivum l. Yangmai 158. The expression of the TaHBP1 in powdery mildew-susceptible triticum asetivum l. variety Yangmai 158 is induced to enhance by erysiphe cucurbitacearum, and the expression level is much higher than the expression level in disease-resistant triticum asetivum l. variety Nannong 9918. A positive sequence of the TaHBP1 is inserted between BamHI and KpnI digestion sites of pWMB006, and at the same time, a reverse sequence is inserted between SpeI and ScI of pWMB006 to obtain interference expression vectors. The powdery mildew-susceptible triticum asetivum l. variety Yangmai 158 is transformed. The identification result of powdery mildew resistance of positive transformed plants shows that the resistance of powdery mildew-susceptible triticum asetivum l. varieties on powdery mildew is improved with decreasing expression of the TaHBP1.

The present invention relates to methods for controlling phytopathogenic fungi using biological control agents. More specifically, the invention relates to methods for controlling phytopathogenic fungi having a long incubation period. In particular, the methods according to the invention are particularly suited for controlling the causal agent of powdery mildew on grapes, the fungus Erysiphe necator.

The invention provides a rubber tree erysiphaceae avirulence gene screening method, effect protein and application. The screening method comprises the following steps: (1) selecting a coding gene A of potential effect protein from rubber tree erysiphaceae genes; (2) constructing a rubber tree erysiphaceae effect protein expression vector, and expressing and transforming a potential effect protein gene A of erysiphaceae in colletotrichum gloeosporioides to obtain a colletotrichum transformant; and (3) culturing the colletotrichum transformant with high expression degree of the effect protein, inoculating the colletotrichum transformant to an in-vitro rubber leaf, determining the pathogenic ability of the colletotrichum transformant, and if the pathogenicity of the colletotrichum gloeosporioides transformant is reduced compared with that of wild colletotrichum gloeosporioides, indicating that the effect protein coding gene A is an avirulence gene. According to the screening method disclosed by the invention, the erysiphaceae avirulence gene can be directly screened through pathogenicity of protein gene transformants with different effects, so that the avirulence gene of the erysiphaceae of the rubber tree can be more quickly screened and identified, and prevention and control of rubber erysiphaceae and breeding of disease-resistant varieties can be enhanced.

Provided herein are Vitis vinifera exhibiting Erysiphe necator resistance. In particular, provided herein are Vitis vinifera having in their genome mildew resistance locus O (MLO) genes, in particular an MLO7 gene and an MLO6 gene, where the MLO7 gene and MLO6 gene have reduced expression and/or function.

The application discloses a bacterial strain for preventing and treating preventing and treating erysiphe cichoracearum of tobacco and a fermentation method thereof, and a compounding agent for preventing and treating the erysiphe cichoracearum of the tobacco. The bacterial strain is a bacterial strain WSWFJ45; and the bacterial strain is bacillus amyloliquefaciens, and the preservation registration number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.20148. The bacterial strain is applied to the agricultural production process and can effectively prevent and control the erysiphe cichoracearum of the tobacco. After a bacterial strain propagation material is compounded with a medium-low toxicity chemical pesticide, the dosage of the chemical pesticide can be effectively reduced, the dosage of the pesticide is only half of the normal dosage, and the use of the low toxicity chemical pesticide can be reduced to a great extent, so that the environmental pollution is reduced, and the safety of crops is improved; and meanwhile, through detection, the prevention and treatment effect of the compounding agent is superior to that of the chemicalpesticide merely used. The bacterial strain can effectively prevent and control the erysiphe cichoracearum of the tobacco, the application amount of the chemical pesticide can be reduced to a great extent, environmental pollution is reduced and the safety of the crops is improved.

The invention provides an amide compound and a preparation method and application thereof. The amide compound is provided with a structure shown in the formula I, has a remarkable effect on controlling diseases in agriculture and forestry and particularly has a good control effect on erysiphe cichoracearum, pseudoperonospora cubensis and phakopsora pachyrhizi; when the concentration of the amide compound is 400ppm, the control effect on the erysiphe cichoracearum is greater than or equal to 90%, and the control effect on the pseudoperonospora cubensis is greater than or equal to 90%; when theconcentration of the amide compound is 100ppm, the control effect on the phakopsora pachyrhizi is greater than or equal to 90%; and moreover, the preparation method of the amid compound is simple andefficient, large-scale production is easy, and application prospects are broad.

The invention discloses a wild vitis quinquangularis shang-24 disease-resistant gene VqWRKY31 and application thereof. The full length of a complete open reading frame sequence of the gene is 1608bp, and 536 amino acids are encoded. According to the invention, a pCAMBIA2300-35s-VqWRKY31-3*Flag overexpression vector is constructed, and the overexpression vector is stably transferred into Thomson seedless white grapes by an agrobacterium-mediated grape genetic transformation method. The result shows that the powdery mildew resistance of a transgenic plant overexpressing VqWRKY31 is higher than that of wild grape, and the combined analysis of metabolome and transcriptome shows that the gene is mainly used for biosynthesizing the expression of key genes by regulating stilbene compounds and flavonoids. Furthermore, synthesis of substances such as delphinidin-3-O-glucoside, dihydroresveratrol, hesperetin, cinnamic acid, eriodictyol, resveratrol, ampelopsin F and epsilon-viniferin in the grapes is promoted, so that the biological function of the grapes on powdery mildew resistance is improved.

The disclosure provides a plant of variety NUN 53050 CUP as well as seeds and plants and fruits thereof. NUN 53050 CUP is a parthenocarpic pickling cucumber variety comprising resistance to Cladosporium cucumerinum (Cucumber Scab) and Erysiphe chicoracearum (Powdery Mildew).