34 results about "Erysiphe necator" patented technology

The invention discloses a method for rapidly identifying disease resistance by inoculating grape powdery mildew to detached grape leaves. According to the technical scheme, the method comprises the following steps: separating grape powdery mildew strain microspecies, purifying and infecting; performing rDNA sequence amplification and evolution analysis so as to obtain a grape powdery midew NAFU1 with high pathogenicity; analyzing the preservation and the expanding propagation of the strain; inoculating the strain with the detached grape leaves, dyeing by using trypan blue, inoculating to sick leaves in different periods, and observing indexes such as the time of spore germination, growth velocity, hypha length and necrocytosis, thereby rapidly and accurately detecting the disease resistance of grape. The grape powdery mildew preserved and amplified by using the method is high in sporulation quantity, the spore is fresh and high in pathogenicity, the defects that the conventional field identification period is long, the repeatability is poor and the weather influence can be caused are overcome, not only is the efficiency in detecting the disease resistance of a grape germplasm resource in large scale improved, but also direct reference values in rapidly identifying the disease resistance of the grape germplasm resource as well as hybrid progeny and transgenosis plants of the grape germplasm resource in large scale are achieved.

The present invention relates to powdery mildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei, resistant carrot plants or Daucus carota plants, wherein the powdery mildew resistance is provided by one or two dominant powdery mildew resistance genes. The present invention further relates to molecular markers genetically linked to the present powdery mildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei, resistance providing genes and the use thereof for identifying carrots plants, or Daucus carota plants, being resistant to powderymildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei. The present invention also relates to seeds, plant parts, pollen, egg cells, callus, suspension culture, somatic embryos and edible plant parts of the present plants.

The invention discloses a wild vitis. quinquangularis anti-disease gene and application thereof. The full length of a complete open reading frame sequence is 1095bp, and 364 amino acids are encoded. Moreover, a pCAMBIA2300-35S-VqWRKY52 over-expression vector is constructed, a model plant, namely, arabidopsis thaliana is imported by an inflorescence infection method, and over-expression is performed in the arabidopsis thaliana, so that the resistance of the arabidopsis thaliana to powdery mildew and pseudomonas syringae parasitic in living organisms is improved remarkably, and the resistance to saprophytic botrytis cinerea is lowered. A wild vitis. quinquangularis-24 anti-disease gene VqWRKY52 participates in an SA-mediated anti-disease signal path, can enhance the anaphylactic reaction, can be used for enhancing the resistance of plants to the fungus powdery mildew and the pseudomonas syringae parasitic in the living organisms, and plays a role in regulating and controlling the anaphylactic reactions of the plants through regulation and control of the expression of the VqWRKY52 gene, so that the reactions of the plants to different pathogenic bacteria are regulated and controlled.

The invention discloses a Sporospora 17wyl, its microbial preparation and its application in preventing and treating wheat powdery mildew, aiming to solve the technical problem of chemical control hazards. The preservation number of the bacteria is CGMCC No: 14148, and its microbial preparation contains Sporospora sp. 17wy1 or / and its metabolites; the preparation method of said microbial preparation includes (1) preparation of seed culture; (2) preparation of fermentation broth; (3) Preparations. When the microbial preparation containing Sporospora saccharomyces 17wy1 or / and its metabolites is used in inhibiting the germination of wheat powdery mildew conidia and its growth and reproduction, it has an antagonistic effect on the germination and growth of wheat powdery mildew conidia, and has an antagonistic effect on the germination and growth of wheat powdery mildew conidia. Powdery mildew on both in vitro and in vivo wheat leaves had good control effects. The raw material used in the present invention has low cost, is safe to the environment, and avoids hidden dangers of agricultural safety.

Provided herein are Erysiphe necator resistance conferring genes, plants, plant parts and seeds comprising the present resistance providing genes and the use thereof for selecting Erysiphe necator resistant plants. Specifically, provided herein are Erysiphe necator resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring genes is the primary amino acid sequence represented SEQ ID No. 1, or a primary amino acid sequence with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity with SEQ ID No. 1; and wherein said resistance conferring gene is impaired.

The invention discloses applications of tobacco Mildew resistance locus O (MLO) genes MLO2, MLO6 and MLO12 in the preparation of tobacco varieties resistant to powdery mildew, and a method for preparing the tobacco varieties resistant to powdery mildew. Through the construction of an editing inactive carrier containing polycistron tRNA-gRNA editing MLO2, MLO6 and MLO12 genes, tobacco can show obvious resistance on powdery mildew after the MLO2, MLO6 and MLO12 genes are inactivated by editing; and research results have important application values on researching the anti-disease immune mechanisms of plant on fungi and cultivating the tobacco varieties resistant to the powdery mildew.

The invention discloses a cutting seedling identifying method for muskmelon powdery mildew resistance. The method comprises the following steps: firstly, cutting seedlings with relatively large rooting amount and high survival rate are obtained by adopting muskmelon lateral branch cutting, then a powdery mildew spore suspension liquid spraying method is adopted for carrying out inoculation on thecutting seedlings, and the cutting seedlings are cultured in a high-humidity environment and are subjected to the resistance identification, and the identification result is consistent with an outdoorfield disease-resistant and inoculation experiment result. According to the method disclosed by the invention, the powdery mildew resistance of the muskmelons can be identified under the condition that the environment conditions are controllable, the method is advantageous in that the operation is simple and feasible, and the form is visual and reliable, the period is short, the muskmelon plantsare not damaged, and the method has important significance for resistance heredity on powdery mildew resistance of the muskmelons and breeding research.

The invention belongs to the field of genetic engineering, and discloses a gene TaHBP1 with a heme-binding (Heme-binding) domain, its recombination interference carrier and its application. The cDNA sequence of the gene TaHBP1 with Heme-binding domain is SEQ ID NO.1 and the encoded amino acid sequence is SEQ ID NO.2. The gene is from common wheat (Triticum asetivum L.) Yangmai 158. The expression of TaHBP1 in powdery mildew-susceptible wheat variety Yangmai 158 was induced by powdery mildew, and the expression level was much higher than that in disease-resistant wheat variety Nannong 9918. The forward sequence of TaHBP1 was inserted between the BamHI and KpnI restriction sites of pWMB006, and the reverse sequence was inserted between SpeI and SacI of pWMB006 to obtain an interference expression vector, and the wheat variety Yangmai 158 susceptible to powdery mildew was transformed, and the positively transformed plants The powdery mildew resistance identification results showed that the reduced expression of TaHBP1 could improve the resistance of powdery mildew susceptible wheat cultivars to powdery mildew.

The invention discloses a kinase gene LeRLK1-V, and an expression vector and application thereof, belonging to the field of genetic engineering. The cDNA sequence of LeRLK1-V is SEQ ID No. 1, and an amino acid sequence coded by LeRLK1-V is SEQ ID No. 2. The gene LeRLK1-V is derived from the 6VS chromosome arm of the Triticum aestivum-Haynaldia villosa translocation line 6VS / 6AL Nannong 9918. LeRLK1-V is enhanced in expression under the induction of powdery mildew in the powdery-mildew-resistant wheat variety Nannong 9918. The gene is used for transforming the susceptible wheat variety Yangmai158 by transient expression, and results show that the overexpression of LeRLK1-V can reduce the haustorium index of Yangmai 158. Therefore, LeRLK1-V is expected to be applied to genetic engineering breeding; and as LeRLK1-V is introduced into wheat varieties susceptible to powdery mildew, the powdery mildew resistance of the wheat varieties is expected to be improved.

The invention discloses a chloroplast positioning gene ToxABP1‑V and its application. The cDNA sequence of ToxABP1‑V is SEQ ID NO.1 and the encoded amino acid sequence is SEQ ID NO.3. The gene comes from diploid Trichopyrhiza, can interact with powdery mildew resistance-related gene CMPG1‑V, and its expression is down-regulated by powdery mildew-resistant diploid Trichopyrhiza. The gene RNAi silencing vector pWMB006‑ToxABP1 was transformed into the susceptible wheat variety Yangmai 158 by single-cell transient silencing technology, and the results showed that transient silencing of ToxABP1 could reduce the haustoria index of Yangmai 158. Three positive RNAi transgenic plants were obtained by transgenic technology, which showed medium to high resistance to wheat powdery mildew. Therefore, ToxABP1‑V is expected to be used in genetic engineering breeding.

The invention discloses a spray vaccination method for Blumeria graminis f.sp.tritici and belongs to the technical field of crop pathogenic bacterium vaccination. The spray vaccination method comprises the following steps: (1) expanding propagation of monospora strains; (2) collection of conidia; (3) conidial powder suspension by use of C5-18-perfluorinated alkyl (FluorinertTM FC-40), and preparation of an erysiphe spore suspension with 106 spores / ml; and (4) spray vaccination. Uniform spray vaccination of Blumeria graminis f.sp.tritici is guaranteed, accurate quantification is ensured, and such shortcomings as nonuniform vaccination, accurate quantification failure and the like of a shaking method are overcome to guarantee normal implementation of Blumeria graminis f.sp.tritici related study works. The spray vaccination method can be popularized in Blumeria graminis f.sp.tritici resistance identification and breeding units.

The invention discloses a fungus of the genus Diptera in plants ( Seimatosporium sp.) M7SB 41, the preservation number of which is CGMCC NO.18114 in the General Microorganism Center of the China Microbiological Culture Collection Management Committee; the strain can be applied to the prevention and treatment of tobacco powdery mildew. Erysiphe cichoracearum Tobacco powdery mildew caused by DC) has good antagonism, can significantly promote the growth of infected tobacco, and improve its disease prevention and resistance ability; the present invention has broad application prospects in the field of biological control of powdery mildew, and is a biological control agent for powdery mildew The development and application of the strain provide strain resources and theoretical basis.

The invention discloses a haynaldia villosa agglutinin receptor-like kinase gene, its expression vector and an application thereof, and belongs to the field of gene engineering. According to the invention, cDNA sequence of Hv-LecRK is SEQ ID NO.1 and amino acid sequence coded by Hv-LecRK is SEQ ID NO.2. The gene is derived from Haynaldia villosa L.(2n=2x=14, genome VV), and can interact with powdery mildew resistance-related gene Hv-CMPG. Expression of the gene is enhanced when the gene is induced by powdery mildew in the powdery mildew resistant diploid haynaldia villosa. Through transient expression, the gene is transformed to a susceptible wheat variety Yangmai 158. It shows through results that overexpression of the Hv-LecRK gene can reduce haustorium index of Yangmai 158. Thus, the Hv-LecRK is expected to be used in genetic engineering breeding. By introducing the Hv-LecRK into a wheat variety susceptible to powdery mildew, powdery mildew resistance of wheat is expected to be raised.

The present invention relates to powdery mildew and in particular to powdery mildew, resistant carrot plants or wild carrot (Daucus carota) plants caused by the phytopathogen Erysiphe heraclei, wherein the powdery mildew resistance is controlled by one or two dominant powdery mildews disease resistance genes. The present invention also relates to molecular markers genetically linked to the powdery mildew of the invention and in particular to powdery mildew caused by the phytopathogen Erysiphe miltiorrhiza, genes conferring resistance and their use for identifying tolerant powdery mildew and in particular by the phytopathogen Use of carrot plants or wild carrot plants with powdery mildew caused by Erysipha lovii. The invention also relates to seeds, plant parts, pollen, egg cells, callus, suspension cultures, somatic embryos and edible plant parts of the plants of the invention.