The invention discloses a method for rapidly identifying
disease resistance by inoculating grape
powdery mildew to detached grape leaves. According to the technical scheme, the method comprises the following steps: separating grape
powdery mildew strain microspecies, purifying and infecting; performing rDNA sequence amplification and evolution analysis so as to obtain a grape powdery midew NAFU1 with high
pathogenicity; analyzing the preservation and the expanding propagation of the strain; inoculating the strain with the detached grape leaves,
dyeing by using
trypan blue, inoculating to sick leaves in different periods, and observing indexes such as the time of
spore germination,
growth velocity,
hypha length and necrocytosis, thereby rapidly and accurately detecting the
disease resistance of grape. The grape
powdery mildew preserved and amplified by using the method is high in sporulation quantity, the
spore is fresh and high in
pathogenicity, the defects that the conventional field identification period is long, the
repeatability is poor and the weather influence can be caused are overcome, not only is the efficiency in detecting the
disease resistance of a grape
germplasm resource in large scale improved, but also direct reference values in rapidly identifying the disease resistance of the grape
germplasm resource as well as
hybrid progeny and transgenosis plants of the grape
germplasm resource in large scale are achieved.