Rubber tree erysiphaceae avirulence gene screening method, effect protein and application

Abstract

The invention provides a rubber tree erysiphaceae avirulence gene screening method, effect protein and application. The screening method comprises the following steps: (1) selecting a coding gene A of potential effect protein from rubber tree erysiphaceae genes; (2) constructing a rubber tree erysiphaceae effect protein expression vector, and expressing and transforming a potential effect protein gene A of erysiphaceae in colletotrichum gloeosporioides to obtain a colletotrichum transformant; and (3) culturing the colletotrichum transformant with high expression degree of the effect protein, inoculating the colletotrichum transformant to an in-vitro rubber leaf, determining the pathogenic ability of the colletotrichum transformant, and if the pathogenicity of the colletotrichum gloeosporioides transformant is reduced compared with that of wild colletotrichum gloeosporioides, indicating that the effect protein coding gene A is an avirulence gene. According to the screening method disclosed by the invention, the erysiphaceae avirulence gene can be directly screened through pathogenicity of protein gene transformants with different effects, so that the avirulence gene of the erysiphaceae of the rubber tree can be more quickly screened and identified, and prevention and control of rubber erysiphaceae and breeding of disease-resistant varieties can be enhanced.

Description

Technical field

[0001] The present invention relates to the technical field of microbial gene, and Background technique

[0002] Polyfung fungi is a pathogenic fungi of plant white powder disease, which can cause serious production losses on important food crops and custries. Rubber tree white powder is a common leaf disease in rubber trees, rubber tree white powder infected important economic crops, Brazilian rubber trees, affecting the growth of rubber trees, causing natural rubber production significantly decline. At present, the research on the genetic diversity of rubber trees is still less.

[0003] Non-toxic genes are generally encoded as proteins that have triggered plants resistant reactions in pathogen. In the wheat white powder, white powder has been identified as a special living body parasitic, and a non-toxic gene of a variety of wheat white powder is discovered, which has great significance for the breeding of crops. However, the non-toxic gene of rubber tree white...

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