A cutting seedling identification method for resistance to powdery mildew of melon

Abstract

The invention discloses a cutting seedling identifying method for muskmelon powdery mildew resistance. The method comprises the following steps: firstly, cutting seedlings with relatively large rooting amount and high survival rate are obtained by adopting muskmelon lateral branch cutting, then a powdery mildew spore suspension liquid spraying method is adopted for carrying out inoculation on thecutting seedlings, and the cutting seedlings are cultured in a high-humidity environment and are subjected to the resistance identification, and the identification result is consistent with an outdoorfield disease-resistant and inoculation experiment result. According to the method disclosed by the invention, the powdery mildew resistance of the muskmelons can be identified under the condition that the environment conditions are controllable, the method is advantageous in that the operation is simple and feasible, and the form is visual and reliable, the period is short, the muskmelon plantsare not damaged, and the method has important significance for resistance heredity on powdery mildew resistance of the muskmelons and breeding research.

Description

technical field [0001] The invention belongs to the technical field of plant disease-resistant breeding, and in particular relates to a cutting seedling identification method for powdery mildew resistance of muskmelon. Background technique [0002] Melon fruit is crispy, sweet, unique in flavor and rich in nutrients. It is a commercial crop widely planted in the world and has become a high-grade melon and fruit popular among consumers. However, the main cultivars of muskmelon are extremely vulnerable to various pathogens in production, especially powdery mildew, which is the most serious and widely distributed. It has become the main disease of muskmelon worldwide and the main obstacle to green production of muskmelon. The main damage part of powdery mildew is the leaves of muskmelon plants. After the pathogen has an affinity reaction with muskmelon, it obtains nutrients from the host through the formation of haustoria, so that the infected parts become chlorotic and dry, wh...

AI Technical Summary

Problems solved by technology

The spray inoculation method at the seedling stage has relatively strict requirements on the disease conditions, and the method is cumbersome, the identification period is long, time-consuming and laborious, and wastes seed materials, especially not suitable for the identification of disease resistance of rare melon germplasm resources

Similarly, the isolated leaf inoculation method requires aseptic operation on a solid medium, and the cost is relatively high, and it is impossible to identify the disease resistance of a large number of germplasm resources.

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