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A Plant Endophytic Trichotheca fungus m7sb 41 and Its Application

An endophytic fungus, double-ended technology, applied in fungi, microorganisms, microorganisms, etc., can solve the problems of less research on endophytes and no reports on the application of fungi of the genus Trichospora spp. to improve disease resistance, Low cost, easy bacteria effect

Active Publication Date: 2021-09-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on endophytes in the control of tobacco powdery mildew
At present, there are no reports on the application of Trichosporum fungus in the control of tobacco powdery mildew at home and abroad

Method used

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  • A Plant Endophytic Trichotheca fungus m7sb 41 and Its Application
  • A Plant Endophytic Trichotheca fungus m7sb 41 and Its Application
  • A Plant Endophytic Trichotheca fungus m7sb 41 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation, screening and identification of Trichoderma discidioides fungus M7SB 41

[0027] 1. Collect plant samples

[0028] The powdery mildew-resistant wild rose Dali Zihua and the powdery mildew-susceptible wild rose seven sisters were collected from the Cangshan area of ​​Dali City, Yunnan Province; the samples were collected in an S-shape, and three replicates were set; the specific sampling method was: cut with sterile scissors Twenty leafy branches with a length of about 0.5 m were placed in sterile bags immediately after collection, labeled, brought back to the laboratory and the isolation of endophytes was completed within 24 hours after sampling;

[0029]2. Rinse the plant samples collected above in tap water, divide them into stems and leaves, and carry out surface disinfection: first, soak in 75% ethanol solution by volume for 3 minutes, rinse with sterile water for 4 times, and then use available chlorine concentration Soak in 5% sodium hypochl...

Embodiment 2

[0048] Example 2: Control of Tobacco Powdery Mildew by M7SB 41

[0049] The present embodiment is intended to prove the control effect of Trichothetia spp. M7SB 41 on the tobacco powdery mildew caused by bisporus powdery mildew, and its experimental process is as follows:

[0050] A. Preparation of Bacteroides spp. strain M7SB 41 bacterial suspension: Inoculate s. spp. M7SB 41 on the PDA plate medium, cultivate at 25°C for 10 days, and use a sterile scalpel along the surface of the plate. Scrape the mycelium, take 3g of the mycelium, cut it into pieces with sterile surgical scissors, add it into 20mL of sterile water, mix well, and make a M7SB 41 bacterial suspension of 15g / 100mL of live mycelium for later use;

[0051] B. Preparation of powdery mildew spore suspension: use sterile scissors to cut tobacco K326 tobacco leaves infected with powdery mildew. spare;

[0052] C. Greenhouse potted plant control effect experiment: Tobacco K326 seeds were purchased from Yuxi China To...

Embodiment 3

[0055] Example 3: The effect of inoculation plate Dichothecosis sp. M7SB 41 on the growth of diseased tobacco

[0056] The experimental process and technical method are the same as in Example 2, the difference is that while observing the incidence of powdery mildew, the determination of the biomass of tobacco seedlings is increased; that is, the control group and the treatment group are measured on the 7th day of spraying the powdery mildew spore suspension. Chlorophyll content of plants; tobacco seedlings were harvested on the 14th day after spraying powdery mildew spore suspension, and biomass such as plant height, root length and dry weight were measured.

[0057] The results of the pot experiment are shown in Table 1 and Figure 4 As shown, the inoculated plate Dichothes sp. strain M7SB 41 has a significant promoting effect on the growth of tobacco seedlings infected with powdery mildew; However, the increase in root length was not significant ( P > 0.05), while the incr...

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Abstract

The invention discloses a fungus of the genus Diptera in plants ( Seimatosporium sp.) M7SB 41, the preservation number of which is CGMCC NO.18114 in the General Microorganism Center of the China Microbiological Culture Collection Management Committee; the strain can be applied to the prevention and treatment of tobacco powdery mildew. Erysiphe cichoracearum Tobacco powdery mildew caused by DC) has good antagonism, can significantly promote the growth of infected tobacco, and improve its disease prevention and resistance ability; the present invention has broad application prospects in the field of biological control of powdery mildew, and is a biological control agent for powdery mildew The development and application of the strain provide strain resources and theoretical basis.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a fungus of the genus Trichospora ( Seimatosporium sp.) M7SB 41 and its application. Background technique [0002] Tobacco powdery mildew (Tobacco Powdery Mildew) is one of the main diseases of tobacco leaf production, which is caused by powdery mildew ( Erysiphe cichoracearum DC.) fungal disease caused by infection, which occurs nationwide. The lesion usually starts from the lower leaves and spreads from bottom to top. In severe cases, all the leaves fall off, leaving only the veins (Guo Qitang et al., 2009). The diseased leaves are brown in color after baking, with obvious punctate lesions on the leaf surface, thin as paper, less oil, severe lack of aroma, heavy miscellaneous gas, large damage, high crushing rate, which seriously affects the yield and quality of tobacco leaves ( Liu Caiyun et al., 2018). At present, cultivating dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00
CPCC12N1/145C12R2001/645
Inventor 李海燕赵祎朱政清汤雯婷李绍仕
Owner KUNMING UNIV OF SCI & TECH
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