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Saccharomyces 17wy1, its microbial preparation and its application in the control of wheat powdery mildew

A microbial preparation and yeast technology, applied in its microbial preparation, application in the prevention and control of wheat powdery mildew, and the field of Sporospora xylophilus 17wy1, can solve the problems of high toxicity, large environmental impact, poor stability, etc., to reduce the degree of morbidity , good antagonistic effect, easy to produce effect

Active Publication Date: 2020-12-18
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many bacteria or molds or their metabolites with bactericidal activity have been found, they have not been formulated for production due to their high toxicity to humans, animals, fish or natural enemies, poor stability, and large impact on the environment.

Method used

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  • Saccharomyces 17wy1, its microbial preparation and its application in the control of wheat powdery mildew
  • Saccharomyces 17wy1, its microbial preparation and its application in the control of wheat powdery mildew
  • Saccharomyces 17wy1, its microbial preparation and its application in the control of wheat powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Medium formulation

[0031] PDA medium: potato 200 g / L, glucose 20 g / L, agar 20 g / L, natural pH, sterilized by high temperature and high pressure at 121 °C for 30 min.

[0032] YPD medium: yeast extract 10 g / L, peptone 20 g / L, glucose 20 g / L, natural pH, sterilized by high temperature and high pressure at 121 °C for 30 min.

Embodiment 2

[0033] Example 2: Saccharomyces sp. ( Tilletiopsipallescens ) isolation, purification and identification

[0034] (1) Take abnormally growing wheat leaves, such as powdery mildew with scattered conidia, accumulation of yellow-brown secretions on leaves, slow growth of leaves and dying;

[0035] (2) Carefully scrape it with a sterilized scalpel and inoculate it on the PDA solid medium, and cultivate it for 3 to 5 days at a constant temperature of 25 °C;

[0036] (3) Pick the part with mapped colonies, reversely connect it to PDA medium for purification, repeat twice;

[0037] (4) Observe the morphological characteristics of cells under a high-power microscope;

[0038] (5) Collect the hyphae in PE centrifuge tubes, extract DNA with OMEGA kit, and amplify the ITS sequence by PCR.

[0039] The results show:

[0040] Sporocyst is a single cell that reproduces asexually in the form of budding and can eject spores, characterized by the formation of red or pink colonies and kidn...

Embodiment 3

[0045] Example 3: Inhibition of the germination and growth and reproduction of wheat floury mildew conidia by Sporomyces sp.

[0046] (1) Pick 17wy1 of Saccharomyces sp. grown on the PDA solid medium, inoculate it in the prepared YPD liquid medium, and place it in a shaker at 180rpm and 25°C for 5 to 7 days. The filtrate after the bacteria are released;

[0047] (2) Prepare 1% water agar, dissolve it in a microwave oven, and dilute the filtrate to 10 -1 , 10 -2 times, the sporozoite filtrate was divided into stock solution, 10 -1 , 10 -2 and CK (clean water) for 4 treatments, and mix it with unsolidified water agar at a ratio of 1:1 and apply it to a clean glass slide;

[0048] (3) Take fresh powdery mildew conidia and shake them evenly on the condensed water agar. Incubate in the dark for 24h in a 18°C ​​incubator;

[0049] (4) Microscopic examination under microscope, according to the formula: spore germination inhibition rate=(control germination rate-treatment germinat...

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Abstract

The invention discloses a Sporospora 17wyl, its microbial preparation and its application in preventing and treating wheat powdery mildew, aiming to solve the technical problem of chemical control hazards. The preservation number of the bacteria is CGMCC No: 14148, and its microbial preparation contains Sporospora sp. 17wy1 or / and its metabolites; the preparation method of said microbial preparation includes (1) preparation of seed culture; (2) preparation of fermentation broth; (3) Preparations. When the microbial preparation containing Sporospora saccharomyces 17wy1 or / and its metabolites is used in inhibiting the germination of wheat powdery mildew conidia and its growth and reproduction, it has an antagonistic effect on the germination and growth of wheat powdery mildew conidia, and has an antagonistic effect on the germination and growth of wheat powdery mildew conidia. Powdery mildew on both in vitro and in vivo wheat leaves had good control effects. The raw material used in the present invention has low cost, is safe to the environment, and avoids hidden dangers of agricultural safety.

Description

technical field [0001] The invention relates to the technical field of microbial biological prevention and control, in particular to a kind of Saccharomyces sp. 17wyl, its microbial preparation and its application in the prevention and treatment of wheat powdery mildew. Background technique [0002] By powdery mildew ( Blumeriagraminis (DC.) E.O.Speerf.sp.tritici ) infection caused by wheat powdery mildew is widespread worldwide, which has a serious impact on the yield and quality of wheat, and is one of the main diseases in wheat production. [0003] Chemical control is an important measure for the control of wheat powdery mildew. It has the advantages of good control effect, quick effect, convenient use, less seasonal restrictions, and suitable for large-scale use. However, with the expansion of chemical control in recent years, or abuse, Some disadvantages, such as pollution to the environment and agricultural products, and drug resistance of bacteria are gradually emerg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A01N63/32A01P3/00C12R1/645
CPCA01N63/30C12N1/16C12N1/145C12R2001/645
Inventor 王俊美李亚红徐飞宋玉立韩自行刘露露张姣姣李丽娟赵凯
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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