Building method and mass detection method for fingerprint of total alkaloid components of cortex mori radicis medicinal material or cortex mori radicis extract
A technology of Morus alba extract and construction method, applied in the direction of material separation, measuring device, analysis material, etc., can solve the problems of single component, cannot fully reflect the content of total alkaloid active components in Morus alba, and achieve stability Good, improved quality control standards, good reproducibility
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[0028] (a) Preparation of Cortex Morus alba Medicinal Material Solution: With 0.02mol / L hydrochloric acid solution or water as solvent, the powder of Cortex Morus alba medicinal material is formulated into Cortex Morus alba medicinal material solution, preferably adopting 0.02mol / L hydrochloric acid solution as solvent , because the alkaloid components are generally weakly alkaline, the salt-forming effect in the extraction process with dilute hydrochloric acid can ensure the maximum extraction rate of the alkaloid components. Of course, the purpose of the present invention is to prepare the Cortex Mori medicinal material into a solution that can be used for high-performance liquid chromatography separation, so under this purpose, the solvent used in the present invention is not limited to 0.02mol / L hydrochloric acid solution or water. The concentration of the solution can be determined according to the loading capacity of the chromatographic column used, the flow rate of the m...
Embodiment 1
[0039] Example 1 Establishment of Control Fingerprint of Cortex Morus alba Medicinal Material
[0040] (1) The source of Morus alba medicinal material:
[0041] The 20 batches of Morus alba medicinal materials used in the embodiments of the present invention are mainly produced in Henan, Hebei, Anhui, Guangxi, and Hunan. The above-mentioned varieties have been identified and meet the pharmacopoeia standards without obvious biological differences. See Table 1 for details.
[0042] Table 1
[0043] batch number
place of origin
Are there biological differences
Y070609HN
Henan
none
Y100513HB
Hebei
none
Y100716HB
Hebei
none
Y101113HB
Hebei
none
Y101229HB
Hebei
none
Y110104HN
Hunan
none
Y101230AH
Anhui
none
Y100826HN
Henan
none
[0044] Y101026HB
Hebei
none
Y101102HN
Henan
none
Y110224GZ
...
Embodiment 2
[0053] Example 2 Method for detecting the quality of fingerprints of Morus alba medicinal material
[0054] (1) Preparation of the Morus alba medicinal material solution to be tested: pass the Morus alba medicinal material (batch number Y100513HB) powder through a No. 3 sieve, accurately weigh 0.2g, put it in a 250ml volumetric flask, and add an appropriate amount of 0.02mol / L hydrochloric acid solution , sonicate (power 250W, frequency 28KHZ) for 45 minutes, let cool, add 0.02mol / L hydrochloric acid solution to the mark, shake well, and let stand;
[0055] (2) Preparation of reference substance solution: accurately weigh an appropriate amount of 1-deoxynojirimycin hydrochloride reference substance, add water to make a solution containing 4 μg per 1 ml, and obtain it;
[0056] (3) according to the chromatographic determination method of embodiment 1 and the fingerprint spectrum of measuring the cortex Mori medicinal material to be tested, as Figure 4 shown;
[0057] (4) The...
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