Whole blood urea biosensing test paper

A technology for biosensing and urea, applied in the field of biosensing, can solve problems such as limited application, complexity of composition system, etc., and achieve the effects of convenient blood collection, convenient and fast testing, and simple and fast testing method.

Inactive Publication Date: 2013-02-13
BEIJING SPORT UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The complexity of making this method practically constitutes a system also limits its wide applicability

Method used

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  • Whole blood urea biosensing test paper
  • Whole blood urea biosensing test paper
  • Whole blood urea biosensing test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Use the screen printing method to print the conductive layer of the connecting wire, the conductive layer of the working electrode and the conductive layer of the reference electrode on the PVC substrate with conductive silver paste ink, and print with carbon ink on the conductive layer of the connecting wire, the conductive layer of the working electrode and the conductive layer of the reference electrode Connect the wire carbon coating, the working electrode carbon coating and the reference electrode carbon coating, and then use the mixture of silver paste ink and AgCl to print on the reference electrode carbon coating to form an Ag / AgCl layer. The mixing ratio of silver paste ink and AgCl is 10 / 1 (W / W). Paste two double-sided tapes horizontally on the working electrode and the reference electrode, leaving a gap between the two double-sided tapes to form the channel of the reaction pool. Containing α-ketoglutarate 26.28ug, NADH119.4ug, urease 0.36U, glutamate dehydrog...

Embodiment 2

[0029]Use the screen printing method to print the conductive layer of the connecting wire, the conductive layer of the working electrode and the conductive layer of the reference electrode on the PVC substrate with conductive silver paste ink, and print with carbon ink on the conductive layer of the connecting wire, the conductive layer of the working electrode and the conductive layer of the reference electrode Connect the wire carbon coating, the working electrode carbon coating and the reference electrode carbon coating, and then use the mixture of silver paste ink and AgCl to print on the reference electrode carbon coating to form an Ag / AgCl layer. The mixing ratio of silver paste ink and AgCl is 10 / 1 (W / W). Paste two double-sided tapes horizontally on the working electrode and the reference electrode, leaving a gap between the two double-sided tapes to form the channel of the reaction pool. Will contain α-ketoglutarate 26.28ug, NADH119.4ug, urease 0.36U, glutamate dehydro...

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Abstract

The invention relates to whole blood urea biosensing test paper. The whole blood urea biosensing test paper comprises a substrate, wherein a work electrode, a reference electrode and connecting wires matched with the work electrode and the reference electrode are printed on the substrate; one end of the substrate is printed with an output terminal of the connecting wire to be connected with a test instrument; and reaction layers capable of reacting with urea are printed on the work electrode and the reference electrode. When the whole blood urea biosensing test paper is adopted for testing blood urea, compared with the enzyme kinetics colorimetry method requiring for a semi-automatic or full-automatic instrument in the prior art, testing is more convenient and quick, so that the blood urea index can be more widely applied to sports monitoring or medical analysis, and a simple and quick test method is provided for general people.

Description

technical field [0001] The invention relates to a whole blood urea biosensing test paper, which belongs to the technical field of biosensing. Background technique [0002] The blood urea index can reflect the body’s protein metabolism during exercise, and can be used as a sensitive index for evaluating exercise volume and supervising overtraining. Therefore, it is an extremely important monitoring index both in the process of exercise training and in the evaluation of exercise recovery after exercise. [0003] At present, there are condensation method and enzyme coupling rate method for the determination of blood urea at home and abroad. Among them, the condensation method has poor accuracy, and the enzyme coupling rate method is relatively fast and accurate. These two methods need to go through the process of blood collection, centrifuged serum, reagent sample addition, and colorimetry on the machine, which takes a relatively long time. As some scholars reported, the spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00G01N27/07
Inventor 周越魏晓雨艾磊许岳韩奇何辉熊开宇王瑞元
Owner BEIJING SPORT UNIV
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