Insecticidal composition containing oxymatrine and benzoylurea components
An insecticidal composition and technology of oxymatrine, applied in the direction of insecticides, acaricides, biocides, etc., can solve the problems of synergistic compounding of insecticidal ingredients and application research that have not been reported, and prolong the service life , the effect of delaying the development of resistance
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Embodiment 1
[0014] Biological Example 1: Beet Spodoptera
[0015] Beet armyworm collected in the field was used in the experiment, which was used for the experiment after two generations of breeding. The specific method is as follows:
[0016] Leaf disc preparation: select clean bracts, wash and dry them, and use a puncher to make leaf discs for later use.
[0017] Pharmaceutical preparation: The original drug for the test is provided by the manufacturer, and the required preparation is made by our company's product development center. Before the test, different concentration gradients were prepared according to different proportioning schemes, with 6 concentrations for each proportioning.
[0018] Dipping: Use tweezers to pick up the leaf discs and dip them in the liquid medicine for 5 seconds, take them out and dry them on absorbent paper, and put them in a 24-well plate after air-drying naturally, with 2 pieces per well. A total of 3 plates were placed for each concentration treatme...
Embodiment 2
[0032] Biological Example 2: Tetranychus cinnabarinus
[0033] Insect test: Tetranychus cinnabarinus raised on broad bean seedlings indoors all the year round. Before the test, stick double-sided tape on one end of the glass slide, and use a brush to select the multiplied adult mites of the same age and paste them on the tape. About 50 slices are placed in a 9cm petri dish covered with filter paper for moisturizing. After 2 hours, the microscopic examination was carried out, and the dead worms were discarded.
[0034] Pharmaceutical preparation: The original drug for the test is provided by the manufacturer, and the required preparation is made by our company's product development center. Before the test, different concentration gradients were prepared according to different proportioning schemes, with 6 concentrations for each proportioning.
[0035] Immersion: Start with a low concentration, pick up the glass slide with tweezers, immerse the end with the mite in the soluti...
Embodiment 3
[0042] Biological Example 3: Rice leaf roller
[0043] Test insects: Cut the fresh buds of the rice leaf roller collected in the field to about 8 cm in length, put them into disposable plastic cups, and put 30 to 40 buds in each plastic cup.
[0044] Pharmaceutical preparation: The original drug for the test is provided by the manufacturer, and the required preparation is made by our company's product development center. Before the test, different concentration gradients were prepared according to different proportioning schemes, with 6 concentrations for each proportioning.
[0045] Spray: use a POTT spray tower to spray at a pressure of 50PSI, and spray 2ml each time. The spray starts from low concentration, and the blank control sprays the same amount of water. After spraying, cover the roll with paper, tie it with a rubber band, and put it in the insect culture room (25±1°C, 12H / 12H).
[0046] Survey statistics: Check the number of dead insects after 24 hours of treatme...
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