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Artificially-synthesized G4 phage and applications thereof

An artificial synthesis and phage technology, applied in the field of phage, to prevent the evolution of strains, prevent the generation of drug-resistant strains, and increase the lethality

Inactive Publication Date: 2013-03-27
蔡志明 +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] With the continuous enhancement of microbial resistance, the development of antibiotics has become less and less able to meet the requirements, therefore, the development of a new antibacterial therapy has become more and more urgent

Method used

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  • Artificially-synthesized G4 phage and applications thereof
  • Artificially-synthesized G4 phage and applications thereof
  • Artificially-synthesized G4 phage and applications thereof

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Embodiment Construction

[0018] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0019] The inventor utilizes the existing G4 phage gene ([NCBI Reference Sequence NC_001420.2]) to mutate to obtain the G4 phage gene of the present invention. The DNA sequence of the existing G4 phage gene is the second sequence shown in SEQ ID NO: 2. The present invention The DNA sequence of the G4 phage gene is the first sequence shown in SEQ ID NO:2. The mutation sites of the second sequence are respectively located at the 3016th nucleotide, the 3328th nucleotide and the 3358th nucleotide from the 5' end of the second sequence, and the mutation results are respectively mutated from t to a , from t to c and from t to c.

[0020] Such as figure 1 As shown, the first sequence has EcoR1, Kpn1, and Ncol restriction endonuclease sites, and these three endonuclease sites correspond to the above-mentioned 3328th, 3358th, and 3016th ...

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Abstract

The present invention discloses artificially-synthesized G4 phage and applications thereof. According to the artificially-synthesized G4 phage, a DNA sequence is a first sequence represented by SEQ ID NO:1, the first sequence is obtained through mutation of a second sequence represented by SEQ ID NO:2, mutation points are positioned on 3016th nucleotide, 3328th nucleotide and ??3358th nucleotide on the second sequence from the 5' terminal, and mutation results respectively are mutation of t into a, mutation of t into c, and mutation of t into c. Based on specific bacterial lysis characteristics of the G4 phage, drug resistance Escherichia coli can be killed, and strain evolution and drug resistance strain generation can be prevented so as to effectively increase fatality rate of the Escherichia coli.

Description

technical field [0001] The invention relates to the field of phages, in particular to an artificially synthesized phage. Background technique [0002] With the continuous enhancement of microbial resistance, the development of antibiotics has been increasingly unable to meet the requirements, therefore, the development of a new antibacterial therapy has become more and more urgent. Phages are highly specific to some bacteria. When phages infect bacteria, they can multiply in bacteria and kill bacteria; they are not toxic to animals and plants. Therefore, phages are expected to become better antibacterial drugs due to their unique natural characteristics. [0003] An emerging field of life science research in the "post-genome era" of synthetic biology. This discipline achieves the reconstruction of living systems by rationally and systematically assembling a variety of natural or artificially designed biological components. This technology develops the DNA construction in ...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04A23L3/3571A01N63/00A61P1/00C11D3/38C11D7/40C11D9/38C12R1/92
CPCY02A50/30
Inventor 蔡志明杨锐林唐爱发刘宇辰韩永华叶艺旺段永刚
Owner 蔡志明
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