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A kind of heat-resistant protective agent and its application

A technology of heat-resistant protective agent and protective agent, which is applied in the direction of anti-viral agents, medical preparations with non-active ingredients, and viral antigen components to achieve the effect of enhancing heat-resistant performance and reducing physical damage

Active Publication Date: 2014-10-08
南京国创生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing heat-resistant lyoprotectants can only preserve live vaccines for 24 months at 2-8°C and 30 days at 37°C.

Method used

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  • A kind of heat-resistant protective agent and its application
  • A kind of heat-resistant protective agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of heat-resistant protective agent

[0027] In this embodiment, if there is no special description, the percentages all refer to weight percentages.

[0028] Among them, casein hydrolysate was purchased from ShanHai Richu Bioscience Co.. Ltd, batch number 090901; polyvinylpyrrolidone (K-30) was purchased from Sinopharm Chemical Reagent Co., Ltd., batch number F20111009; Pluronic 68 (Pluronic F 68) was purchased From Shanghai Shenggong Biological Co., Ltd., batch number HJ0522B2012.

[0029] Heat-resistant protective agent 1 formula:

[0030] Trehalose 68%,

[0031] Glycine 6%,

[0032] Gelatin 3%,

[0033] Casein hydrolysate 6%,

[0034] Polyvinylpyrrolidone 6%,

[0035] Glycerol 5%,

[0036] Plunik 68.6%.

[0037] Heat-resistant protective agent 2 formula:

[0038] 38% trehalose, 20% sucrose,

[0039] Arginine, glycine, methionine, glutamic acid and isoleucine each 2%,

[0040] Gelatin 6%,

[0041] Casein hydrolysate 10%,

[0042] Polyvinylpyrrolidone 3%,

[0043] Gly...

Embodiment 2

[0078] Example 2 Preparation of freeze-dried powder of live Newcastle disease vaccine and its heat resistance

[0079] (1) Preparation of heat-resistant protective agent

[0080] According to the method of Example 1, the heat-resistant protective agents 1-6 were prepared.

[0081] (2) Preparation of chicken Newcastle disease virus liquid (live virus liquid)

[0082] The chicken Newcastle disease virus liquid was provided by Nanjing Tianbang Biotechnology Co., Ltd. Specific preparation method: Prepare chicken Newcastle disease virus liquid (La Sota strain) in accordance with the relevant requirements of the "Compilation of Quality Standards for Veterinary Biological Quality Standards" (2006-2008). The virus content of each virus liquid is 10 7.5 EID 50 (Chicken embryo inspection method).

[0083] (3) Preparation of freeze-dried powder of live Newcastle disease vaccine

[0084] Dissolve the heat-resistant protective agent with a solvent to obtain a protective agent solution;

[0085] Mi...

Embodiment 3

[0101] Example 3 Freeze-dried powder of porcine reproductive and respiratory syndrome live vaccine and its heat resistance

[0102] (1) Preparation of heat-resistant protective agent

[0103] According to the method of Example 1, the heat-resistant protective agents 1-6 were prepared.

[0104] (2) Preparation of porcine reproductive and respiratory syndrome virus liquid (live virus liquid)

[0105] The porcine reproductive and respiratory syndrome virus liquid (live virus liquid) is provided by Nannong Hi-Tech Biological Products Co., Ltd. Specific method: According to the relevant requirements of the "Compilation of Quality Standards for Veterinary Biology" (2006-2008), prepare porcine reproductive and respiratory syndrome virus liquid (R98 strain), the virus content of each virus liquid is 10 5.5 TCID 50 (Cell test method).

[0106] (3) Preparation of freeze-dried powder of porcine reproductive and respiratory syndrome live vaccine

[0107] The corresponding porcine reproductive and...

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Abstract

The present invention provides a heat -resistant protective agent and its application, involving the field of living microorganisms.The heat -resistant protective agent is composed of the following substances: 40%to 80%of the low glycogen, 3%to 12%amino acids, 3%to 12%of the gelatin, 3%to 12%of cases of tentinoprotein, and polyethylene climohine 3%~ 12%, 3%to 12%of glycerin, Punnik-683%-12%.The present invention also provides the application of heat -resistant protective agents in preparing live vaccine frozen dried powder.The solvent is dissolved in the heat -resistant protective agent to obtain a protective agent solution; the protective agent solution is mixed with the live virus solution to obtain the live vaccine; the live vaccine is frozen and dry to get the vaccine frozen dried powder.The heat -resistant protective agent of the present invention can improve the heat -resistant performance of the live vaccine. The live vaccine frozen powder prepared by the heat -resistant protective agent is stored at 37 ° C for 2 months, and it is stored for 24 months under 15 ° C.

Description

technical field [0001] The invention relates to the field of preservation of living microorganisms, in particular to a heat-resistant protective agent and its application. Background technique [0002] The heat sensitivity of viruses and other biologically active substances and their related products has always been the bottleneck of long-term storage of live vaccines. my country has a vast territory and great differences in economic levels. The phenomenon of short-term exposure of vaccines to high temperature environments (even above 45°C) still exists during transportation and use. Therefore, higher requirements are put forward for the heat resistance of live vaccines. [0003] The heat-resistant lyoprotectant considers the impact of the physical and chemical changes that may occur in the lyophilized substance on the survival of the live vaccine when the live vaccine is stored at a higher temperature and for a longer storage time. However, the existing heat-resistant lyop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/32A61K47/26A61K47/18A61K47/10A61P31/12A61P31/14A61K9/19A61K39/17A61K39/12
Inventor 卢宇吕芳邓碧华张金秋侯继波
Owner 南京国创生物技术研究院有限公司
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